Bafilomycin A (10ug/mL)
Cat No.: DCP-BMA10UG
Size: 10 mL (10 x 1mL)
Sterile; Ultrapure, Filtered 0.1-micron membrane Once.
Description:
Bafilomycin A (10 µg/mL in DMSO) is a ready-to-use research reagent supplied as a DMSO solution for convenient aliquoting and direct dilution into experimental systems. Bafilomycin A1 is a macrolide antibiotic and potent vacuolar H+-ATPase (V-ATPase) inhibitor that elevates the pH of acidic intracellular compartments by blocking proton translocation, making it a widely used tool in autophagy, lysosome, endosome, and membrane trafficking studies.
Bafilomycin A is a macrolide antibiotic and selective vacuolar ATPase inhibitor that blocks proton transport across intracellular membranes. By preventing acidification of lysosomes and endosomes, it is widely used as a tool compound in cell biology and pharmacology research. The DMSO formulation provides straightforward handling, accurate dosing, and compatibility with small-volume experimental workflows.
Applications
Bafilomycin A1 is frequently used as an autophagy inhibitor to help distinguish between increased autophagosome formation and impaired autophagic degradation. It is also useful in studies of lysosomal function because blocking V-ATPase activity reduces acidification and suppresses the activity of acid-dependent hydrolases. In addition, researchers use it to examine cellular responses to altered vesicular pH, including effects on signaling pathways, intracellular transport, and stress responses.
Quality Control:
- Appearance: Clear, Colorless Liquid.
- Sterility: Filtered 0.1-micron membrane Once.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: -80 ℃ in the dark.
- Shelf life: 1 year (unopened)
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
