Anti-Rad6 antibody, affinity purified (rabbit polyclonal)
Anti-Rad6 antibody, affinity purified (rabbit polyclonal)
General information
| Cat. No. | ：FNK-70-020 |
| Size | ：100 ug |
| Antigen Species | ：Human |
| Host Species | ：Rabbit |
| Purification | ：Affinity purified with the immunogen conjugated to agarose |
| Reactivity | ：Reacts with Rad6A and Rad6B of human mouse, rat and hamster. Not tested in other species |
| Immunogen | ：Human Rad6B full-length recombinant protein |
| Application | ：1. Western blotting (1,000~2,000 fold dilution) ：2. Immunoprecipitation (assay dependent) ：3. Immunofluoscent staining (assay dependent) ：4. Other applications have not been tested |
| Storage | ：Shipped at 4℃. Upon arrival, spin-down and store at -20℃. |
| Form | ：1mg/ml in PBS, 50% glycerol. Filter-sterilyzed. Azide- and carrier-free |
| Data Link | ：UniProtKB/Swiss P49459 (UBE2A/Rad6A_HUMAN), UniProtKB/Swiss P63146 (UBE2B/Rad6B_HUMAN) |
Description
Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120 .
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Fig.1 Western blot analysis of Rad6 in the whole cell extracts of Hela cell (10 ug) with anti-Rad6
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Fig. 2 Rad6 protein in GM637 cells were stained by indirect immunostaining method with the Rad6 antibody.
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References
- Masuyama S. et al. “Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions” Genes to Cells 10: 753-762 (2005) PubMed 16098139 WB, IF
Aliases for UBE2A Gene
- Ubiquitin Conjugating Enzyme E2 A 2 3 5
- HHR6A 2 3 4
- RAD6A 2 3 4
- Ubiquitin-Conjugating Enzyme E2 A 3 4
- E2 Ubiquitin-Conjugating Enzyme A 3 4
- Ubiquitin-Conjugating Enzyme E2A 2 3
- Ubiquitin Carrier Protein A 3 4
- Ubiquitin-Protein Ligase A 3 4
- RAD6 Homolog A 3 4
- UBC2 2 3
- HR6A 2 4
- Ubiquitin-Conjugating Enzyme E2A (RAD6 Homolog) 2
- EC 6.3.2.19 51
- EC 2.3.2.23 4
- MRXS30 3
- MRXSN 3
- UBE2A 5
