Anti-HIV-1, Gag p55, Rabbit-Poly

HIV-1 Gag p55 is a precursor protein of several proteins that form the core structure of AIDS virus, indispensable to its reproduction. p55 is digested by HIV-1 protease, first into intermidiate products p41 and p15. Then p41 is digested into nucleocapsid protein p7 and to p6 and p1, both of unknown function (1)

The product is prepared by immunizing rabbit with recombinant p55 which was over-expressed in E. coli with a plasmid carrying the Gag p55 coding region of HIV-1 virus, and highly purified by several steps of chromatography (2).

Using this antiserum in Western blotting, the bands of 55 kD for HIV-p55, bands of 15kD, 17kD and 24 kD were observed in the extract of the AIDS virus infected cells (Fig. 1).
 

1) It can be used in Western blotting or ELISA for HIV-1 p55 detection and trtration.

 

Fig.1 Detection of HIV-1 p55 by Western blotting using the anti p55 antibody  Lane 1: Extract of MT4 cells Lane2: Extract of MT4 cells infected with HIV-1(LAI strain).  The antiserum was diluted 2,000 fold before use.