Reconstitution Solution
Cat. No. DCP-RS2X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Reconstitution is the process of adding a liquid diluent to a powder to create a usable solution. In this specific case, the reconstitution solution contains NaCl, KCl, dextrose, HEPES, MgCl2, and CaCl2, adjusted to a pH of 7.3. Sodium chloride provides osmotic balance, potassium chloride maintains proper ionic strength, dextrose serves as an energy source for cells, HEPES acts as a buffering agent to maintain pH, magnesium chloride provides magnesium ions for cellular processes, and calcium chloride supplies calcium ions for cellular functions. This balanced solution is designed to provide optimal ionic conditions and maintain cellular stability. The careful preparation ensures the correct concentration and maintains the integrity of the medication or biological material, with attention to factors such as volume, mixing technique, and storage conditions.
Advantages:
- Ionic Balance and Stability: The combination of sodium chloride (NaCl) and potassium chloride (KCl) helps maintain osmotic balance and proper ionic strength, which are crucial for cell membrane potential and overall physiological stability.
- Energy Provision: Dextrose serves as an energy source, providing essential calories that support metabolic activities. This is particularly beneficial in maintaining cellular energy levels during experimental procedures.
- Buffering Capacity: HEPES is included as a buffering agent to maintain a stable pH of 7.3, which is optimal for most cellular activities.
- Support for Cellular Processes: Magnesium chloride (MgCl2) and calcium chloride (CaCl2) supply essential divalent cations that are vital for numerous cellular processes, including enzymatic reactions and signal transduction.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
125 mM |
| Potassium Chloride |
5 mM |
| Dextrose |
10 mM |
| HEPES |
10 mM |
| MgCl2 |
1 mM |
| CaCl2 |
2 mM |
Quality Control:
- Appearance: Clear solution.
- pH: 7.3
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
-
Storage: 4 ℃
-
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
