Anti-HIV-1, Gag p24, Rabbit-Poly
Product#: FNK-65-004
$346.79
Availability:
Ships in 1-2 Weeks
Anti-HIV-1, Gag p24, Rabbit-Poly
General information
| Cat. No. | ：FNK-65-004 |
| Size | ：100 uL |
| Host Species | ：Rabbit |
| Application |
|
| Storage | ：4℃ |
| Form | ：0.09% sodium azide added to the antiserum |
Description
HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). p24 is used as a marker antigen for observing the patient’s condition after treatment, as it indicates the amount of virus in the blood.
The product is prepared by immunizing rabbit with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B (2), and was highly purified by several steps of chromatography (3, 4).
Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).
Usage
1) It can be used in Western blotting or ELISA for HIV-1 p24 detection and trtration.
 |
Fig.1 Detection of HIV-1 p24 and precursor proteins p55 and p41 by Western blotting using the anti p24 antibody Lane 1: Extract of MT4 cells Lane2: Extract of MT4 cells infected with HIV-1(LAI strain). The antiserum was diluted 2,500 fold before use. |
References
- Freed EO, Virology 251:1-15 (1998) Review
- Adachi A et al, J Virol 59: 284 -291(1986)
- Tanaka N et al, Microbiol Immunol 36:823-831 (1992)
- Saito A et al, Microbiol Immunol 39:473-483 (1995)
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