Anti-HBs S, Mono-2

Cat. No.: FNK-BCL-ABMS-02

Size: 100μg

Storage: 4℃ (for long term storage, -20℃. Avoid freeze and thaw cycles)


Introduction:

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection.




Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection and Pre-S2 is reportedly known to related HBV entry or carcinogenesis.

Specifications:

Source : mouse ascitic fluid, monoclonal
Appearance : liquid form（PBS containing biochemically inactive preservative）

Concentration: 1 mg/mL

Antigen: HBsAg L-protein-ST type (FNK-BCL-AGS-02)

Purification: affinity by Protein G

Usage : ELISA (0.1 to 10μg/mL), Western blot (0.1 to 10μg/mL)


Antigen specificity: These antibodies bind only to S domain and not to Pre-S1 or Pre-S2 domains


Genotype specificity: 


Mono-1 binds to L antigen of genotype A and C, weakly of B, and slightly of D.

Mono-2 is selective to genotype C.


Examples:

Western Blot

Antigen: HBsAg-Lprotein (Product#: FNK-BCL-AGC-01) 10 ng   Primary antibody:   -Lane1: Anti-HBs S protein antibody mono-1 (FNK-BCL-ABMS-01)  at 100 ng/mL  -Lane2: Anti-HBs S protein antibody mono-2 (FNK-BCL-ABMS-02)  at 1 μg/mL  Secondary antibody:  Anti-mouse IgG Rabbit (HRP -labeled)   Detection:  ECL substrate 2 min exposure