Anti-HBs Pre-S1, Poly-3 high affinity

Cat. No.: FNK-BCL-ABP1-03

Size: 100μg

Storage: 4℃ (for long term storage, -20℃. Avoid freeze and thaw cycles)


Introduction:

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection.




Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection and Pre-S2 is reportedly known to related HBV entry or carcinogenesis. Pre-S2 region is known to have variation of amino acid sequence depending on genotypes.

Specifications:

Source : white rabbit

Appearance : liquid form（PBS containing biochemically inactive preservative）

Concentration: 1 mg/mL

Antigen: Pre-S1 peptide with thioredoxin tag (genotype C or D)

Purification: affinity by Protein A

Usage : ELISA (0.1 to 10μg/mL), Western blot (0.1 to 10μg/mL)


Antigen specificity: These antibodies bind only to Pre-S1 domain and not to S or Pre-S2 domains


Genotype specificity: 


Examples:

1. Western Blot

M；Marker  1. Antibody: anti-Pre-S1（Code#: FNKJ-BCL-ABP1-01）  Antigen: HBsAg-L 2. Antibody: anti-Pre-S2（Code#: FNK-BCL-ABP2-01）  Antigen: HBsAg -L 3. Antibody: anti-S antigen（Code#: FNK-BCL-ABPS-01）  Antigen: HBsAg -XT Two bands seen in figure correspond to monomer and dimer of sample antigen.

2. ELISA
 

ELISA condition  Immobilized IgG: anti-Pre-S1antibody (polyclonal, code#: FNK-BCL-ABP1-01) at 10μg/mL  Blocking: k-Block-e (Code#: FNK-BCL-BKHE-01)  Detection IgG: anti-Pre-S1antibody (monoclonal, HRP-labeled): 25ng/mL  Antigen: HBsAg L-protein