SigMax 2Ab-PBS
Cat No.: DCP-SM2AbPBS1X
Size: 100 mL
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.
Description:
SigMax 2Ab-PBS represents a significant advancement in secondary antibody-based immunoassays, utilizing a phosphate-buffered saline foundation to optimize detection sensitivity. This innovative PBS-based solution, enriched with a unique blend of PEG 6000, PVP-40, and BSA, markedly enhances the binding affinity between secondary and primary antibodies. By establishing an optimal microenvironment for antibody interactions, SigMax 2Ab-PBS delivers superior signal amplification while effectively minimizing background interference, ensuring high clarity in multi-step immunodetection protocols. The formulation preserves antibody conformations and reduces non-specific binding, resulting in notably improved assay performance across ELISA, immunohistochemistry, and flow cytometry applications. Additionally, SigMax 2Ab-PBS offers substantial cost efficiency by improving antibody usage and reducing reagent consumption, making it an essential tool for achieving precise and reliable immunological results.
Applications
- Western blotting, especially for phospho-proteins
- Enzyme-linked immunosorbent assay (ELISA)
- Immunohistochemistry (IHC)
- Immunocytochemistry (ICC)
- Flow cytometry
- Phospho-specific antibody applications
Advantages:
- Enhances primary antibody binding efficiency by up to 10-fold
- Ideal for phospho-specific antibodies and assays
- Reduces non-specific binding, improving signal-to-noise ratio
- Compatible with a wide range of primary antibodies
- Allows for lower antibody concentrations, conserving valuable reagents
- Enhances assay sensitivity and specificity in both research and diagnostic settings
- Provides excellent pH stability
Composition:
| Ingredients |
Concentration |
| Sodium Chloride (NaCl) |
137 mM |
| Potassium Chloride (KCl) |
2.7 mM |
| Sodium Phosphate dibasic (Na₂HPO₄) |
10 mM |
| Potassium Phosphate monobasic (KH₂PO₄) |
1.8 mM |
| Polyethylene Glycol 6000 (PEG 6000) |
5.0% (w/v) |
| Glycine |
0.75% (w/v) |
| Triton X-100 |
0.05% (v/v) |
| Tween-20 |
0.1% (v/v) |
| Recombinant Protein G fragments |
0.00005% (w/v) |
| Hydrogen peroxide (H₂O₂) |
0.03% (v/v) |
| Trehalose |
1.0% (w/v) |
| β-Cyclodextrin |
0.1% (w/v) |
| ProClin 300 |
0.05% (v/v) |
Quality Control:
Storage and Shelf Life:
-
Storage: 4℃
-
Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
