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β-1,3-Glucanase B, Thermostable

Product#: FNK-TGL-75-01

$265.30

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β-1,3-Glucanase B, Thermostable

Cat.No. FNK-TGL-75-01
Lot No. 1-T001
Protein conc. 3.0 mg/ml
Volume 1 ml
Form 20 mM Tris-HCl (pH 7.5), 50 mM NaCl
Storage -20 oC
Activity 1089 U/ml
Notes For research use only

Description

EC No.:3.2.1.39 Substrate: β-1,3-glucan Reaction product : β-1,3-glucan oligomer Reaction temperature : ~90℃ Reaction pH : 4.0~8.0
Enzymes work at high temperature with high reactivity
The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

Reaction mix (50 mM Sodium acetate buffer (pH 5.0), 0.5% Laminarin and appropriate amount of the enzyme) was incubated at 85 oC and the reducing power was measured by DNS method (Kongruang et al., (2004), Appl. Biochem. Biotechnol. 113, 213-231). One unit is defined as the amount of enzyme producing reducing power equivalent to 1 ?mol of glucose per 1 minute using Laminarin as a substrate.