ssDNA Quantification Kit, 100 Assays
ssDNA Quantification Kit, 100 Assays
Cat. No.: BM-F1000-SD-100
Description
The Accuris ssDNA Quantification Kit provides an easy and accurate method for the quantification of single-stranded DNA (ssDNA) or oligonucleotides. The assay can detect ssDNA concentrations between 1 and 200 ng. This kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional ssDNA quantification kits. Although the Accuris ssDNA Quantification Kit is not specifically selective for ssDNA over double-stranded DNA or RNA, it is selective for short oligonucleotides. Furthermore, low concentrations of contaminants such as proteins, salts, solvents, and detergents do not interfere with assay sensitivity and accuracy.
The Accuris ssDNA Quantification Kit contains reagent, dilution buffer, and two ssDNA standards. The assay is performed at room temperature — simply prepare the standards and samples, then read on a fluorometer to determine the concentration of your unknown ssDNA samples.
Kit Components & Storage Requirements
| Material | Storage | Amount | Concentration | Stabiliy |
| Accuris dsDNA Reagents (Component A) |
4 ℃ Protect from light |
100 μL | 200X in DMSO | Product Stable for at least 6 months when stored as directed |
| Accuris dsDNA Buffer (Component B) |
20 mL | 1X | ||
| Accuris dsDNA Standard #1 (Component C) |
100 μL | 0 ng/μL in TE Buffer |
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| Accuris dsDNA Standard #2 (Component D) |
100 μL | 100 ng/μL in TE Buffer |
Critical Assay Parameters:
Incubation Time: For optimal detection of ssDNA, incubate the samples and standards with the working solution for 2-3 minutes in the dark at room temperature.
Fluorometer Calibration: Users can either use the previous calibration or perform a new calibration. We recommend performing a new calibration for each assay to minimize deviations in the results.
Assay Temperature: The Accuris ssDNA Quantification Kit offers optimal performance at room temperature. Therefore, all reagents must be equilibrated to room temperature before use.
Effect of Contaminants: The Accuris ssDNA Quantification Kit is tolerant to common contaminants such as proteins, salts, solvents, and detergents (see table below).
Figure: sensitivity of Accuris ssDNA Quantification kits to ssDNA
Standards and Sample Preparation:
Protocol: Using the Accuris ssDNA Quantification Kit with Accuris Smart Q and Qubit Fluorometers
1. Allow all components of the Accuris ssDNA Quantification Kit to reach room temperature.
2. Inspect the Accuris ssDNA reagent for any precipitation. If small precipitates or particles are observed, warm the tube in a water bath until fully dissolved.
3. Prepare the required number of assay tubes, accounting for the two standards. (Use ONLY 0.5 mL thin-wall PCR tubes.)
4. Label the sample and standard tubes on the lids.
5. Prepare the working solution by diluting the Accuris ssDNA reagent 1:200 in 1X Accuris ssDNA Buffer immediately before use. For example, for eight samples plus two standards, dilute 10 μL of Accuris ssDNA Reagent in 1,990 μL of Accuris ssDNA Buffer.
6. Add 10 μL of Standard #1 and Standard #2 to the appropriately labeled tubes (see below).
7. Thoroughly mix the samples and standards, then incubate the tubes at room temperature in the dark for 2-3 minutes
Standards and Sample Measurements
Standard Curve:
1. On the Home screen, select Oligo.
2. Insert standard 1 into a reading chamber and select start symbol followed by pressing the read symbol.
3. After selecting concentration units, choose calibrate followed by Read Standards.
4. Replace standard tube 1 with standard 2 and select Read standards to obtain reading for the second standard.
5. Select Save to save the standard curve. Note: this curve may be re-used for subsequent sample readings. We recommend to prepare new standard curve each time assay is performed.
Sample Quantification:
1. On the fluorometer screen, enter the volume of the unknown sample, then select the Start icon.
2. Once the fluorometer completes the quantification, the sample concentration and Relative Fluorescence Units (RFU) will be displayed on the screen.
3. Remove the sample, insert the next sample into the test chamber, and select the Start icon.
4. Repeat Step 3 until all samples have been quantified. Note: At any point during quantification, the fluorometer screen can be swiped left to display all recorded concentrations from the current experiment, with options to print or export the results. Selecting the Calculator function allows users to calculate the molarity of the sample (note: the ssDNA length [length(bp) = lenght of ssDNA (bases)] must be entered for accurate quantification).
5. After completing the quantification process, turn off the fluorometer
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Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples
- Sensitive quantitation down to 0.1pg/ul ds DNA
- Large color touch screen for programming and operation
- Two models for single or 8-sample processing
- Built-in reagent calculators for sample preparation
- Compact and portable (11 x 6 inch footprint)
Smart-Q Specifications
| Model | Smart-Q™ 100 Fluorometer | Smart-Q™ 800 Fluorometer | Smart-Q™ Mini 115V | Smart-Q™ Mini 230V |
|---|---|---|---|---|
| Display | 7.0-inch, color touch screen | 7.0-inch, color touch screen | No external display (instrument operation via iOS/Android phone or tablet) |
No external display (instrument operation via iOS/Android phone or tablet) |
| Dynamic Range | 5 orders of magnitude | 4 orders of magnitude | 5 orders of magnitude | 5 orders of magnitude |
| Light Source | Blue and Red LED | Blue and Red LED | Blue and Red LED | Blue and Red LED |
| Excitation Wavelengths | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm |
| Emission Wavelengths | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm |
| Stability | ≤ 1.5% | ≤ 1.5% | ≤1.5% | ≤ 1.5% |
| Detector | Photodiodes (300-1000nm) | Photodiodes (300-1000nm) | Photodiodes (320-1100nm) | Photodiodes (320-1100nm) |
| Measurement Speed | ≤ 5 seconds | ≤ 6 seconds | ≤ 5 seconds | ≤ 5 seconds |
| Consumable type | 0.5mL assay tubes | 0.2mL assay tubes (single and 8-tube strip) | 0.5 mL optically-clear quantification tubes | 0.5 mL optically-clear quantification tubes |
| Input Voltage | 24 VDC 2A | 24 VDC 2A | 115V | 230V |
| Dimensions (WxDxH) | 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. | 16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in. | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm |
| Weight | 1.6kg / 3.5lbs | 2.0kg / 4.4lbs | 0.5kg / 1.1lbs | 0.5kg / 1.1lbs |
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Description
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The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
ssDNA Quantification KitThe Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents RNA High Sensitivity Quantification KitThe Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration. This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL RNA Broad Range Quantification KitThe Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification. This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents |
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| Smart-Q 100 for single sample processing | |
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| Smart-Q 800 for up to 8-sample processing | |
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| Accuris DNA Quantification Kits | |
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Ordering Information
| Smart-Q™ | Cat.No. | Products | Qty ( Pack ) | Order |
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BM-F1001 | Smart-Q 100 Fluorometer, single sample | 1 | |
| BM-F1008 | Smart-Q 800 Fluorometer, 8-channel1 | 1 | |
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BM-F0100 | Smart-Q Mini Fluorometer, 115V | 1 | |
| BM-F0100-E | Smart-Q Mini Fluorometer, 230V | 1 | |
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BM-F0100-TAB | Android tablet preloaded with Smart-Q Mini app | 1 | |
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BM-F1000-HS-100 | High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
| BM-F1000-HS-500 | High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-HS1-100 | 1X High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-HS1-500 | 1X High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR-100 | Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-BR-500 | Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR1-100 | 1X Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-BR1-500 | 1X Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-SD-100 | ssDNA Quantification Kit, 100 Assays | 1 Kit | Current page | |
| BM-F1000-SD-500 | ssDNA Quantification Kit, 500 Assays | 1 Kit | |
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| BM-F1000-RHS-100 | High Sensitivity RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RHS-500 | High Sensitivity RNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-RBR-100 | Broad Range RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RBR-500 | Broad Range RNA Quantification Kit, 500 assays | 1 Kit | |
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BM-F1001-T | 0.5mL Fluorescence Detection Tubes, pack of 250 | 250 | |
| BM-F1008-T | 0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000 | 1000 | |
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| BM-F1008-T-8 | 0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120 | 120 | |
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