k-Block-w

Cat. No. FNK-BCL-BKKW-01
Size 500ml
Storage  4°C

Description
Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.

Blocking is very important to get proper results in western blot and ELISA. Beacle has developed four types of ready-to-use blocking solutions each of which has characteristic property.

Features

• Ready-to-Use
• Optimized blocking solution for Western Blot and ELISA
• Pre-coated ELISA plate can be stored for several months.
• b-Block : BSA-based blocking solution. Good for various applications. (Not available)
• h-Block : Casein-based blocking solution. Good for various applications.(Not available)
• k-Block : Degraded casein-based blocking solution. It shows consistent performance in various applications.(Not available)
• c-Block : 100% chemically defined components. Protein free. Best for Western Blot and antibody-detecting ELISA.
• Trial set : Composed of all the above four solutions. Good for screening of most suitable blocking solution in your work. (available c-Block only)

Recommendations for screening best blocking solution
For Western Blot

• To get higher signal (accept some background increase) : k-Block or c-Block
• To reduce background (accept some decrease of signal) : b-Block or h-Block
• Using as a standard blocking : h-Block or b-Block
• Detecting a phospho-protein : c-Block or b-Block

For ELISA

• Antigen-detecting ELISA - Direct method : c-Block , b-Block or k-Block 
  • Sandwich method : k-Block , b-Block or h-Block
• Antibody-detecting ELISA
  • Direct method (detect antibody captured by pre-coated antigen) : c-Block , b-Block or k-Block 
  • Sandwich method : k-Block , b-Block or h-Block

Data
 
Fig. 1 Comparison of blocking solutions M : Marker / SM : 5% Skim Milk / kB : k-Block / hB : h-Block / bB : b-Block / cB : c-Block


 
Fig. 2 Sandwich ELISA Data


 
Fig. 3 Stability data of pre-coated plate