h-Block-w

Cat No. FNK-BCL-BKHW-01

Size:500ml


Cautions
1. Research use only. Do not use for medical purpose.
2. Do not dilute or add other agents in Blocking solutions to get the best result.
3. In some occasions, deposits may occur in solutions. Please dissolve the deposit before use.  


Introduction  
Blocking is very important to get proper results in western blot and ELISA. Beacle has developed four types of ready-to-use blocking solutions each of which has characteristic property.

●Features ●
1．Ready-to-Use
2．Exclusive formulation for Western or ELISA
3．Higher sensitivity than skim milk for Western Products
4．Long-term preservation of pre-coated plate possible by using ELISA blocking solution

• b-Block : BSA-based blocking solution. Good for various applications.
• h-Block : Casein-based blocking solution. Good for various applications.
• k-Block : Degraded casein-based blocking solution. It shows consistent performance in various applications.
• c-Block : 100% chemically defined components. Protein free. Best for Western Blot and antibody-detecting ELISA.

Data

Fig. 1 Comparison of blocking solutions M : Marker / SM : 5% Skim Milk / kB : k-Block / hB : h-Block / bB : b-Block / cB : c-Block

Fig. 2 Sandwich ELISA Data

Fig. 3 Stability data of pre-coated plate
 

Recommendations for screening best blocking solution

For Western Blot

• To get higher signal (accept some background increase) : k-Block or c-Block
• To reduce background (accept some decrease of signal) : b-Block or h-Block
• Using as a standard blocking : h-Block or b-Block
• Detecting a phospho-protein : c-Block or b-Block

• Antigen-detecting ELISA - Direct method : c-Block , b-Block or k-Block
  - Sandwich method : k-Block , b-Block or h-Block
• Antibody-detecting ELISA
  - Direct method (detect antibody captured by pre-coated antigen) : c-Block , b-Block or k-Block
  - Sandwich method : k-Block , b-Block or h-Block