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Tris-based Buffers

Product#: Tris-based Buffers
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verified Tris pKa 8.07 at 25 °C · pH 7–9 Buffering Range

Tris-based Buffers - Category Selection Guide

Tris-buffered saline at three strengths, a Tween-containing TBST, and a sterile Tris-HCl buffer — twelve catalog numbers across five product pages. This page is a category reference; pick the strength and format your protocol needs and click View for its product page.

Tris-based Buffers — Catalog · 12 Catalog Numbers Across 5 Product Pages
This page is a category reference, not a product page. Catalog numbers are grouped under the product page they order from; click View once per group.
Cat. No. Format Size
Tris Buffered Saline TBS, 1X (Tris Buffered Saline), Non-Sterile 1X · 3 catalog numbers · 500 ml · 1000 ml · 10 L
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DCP-TBS1X_500ml 1X 500 ml
DCP-TBS1X_1000ml 1X 1000 ml
DCP-TBS1X_10 Liter 1X 10 L
Tris Buffered Saline TBS, 10X (Tris Buffered Saline), Non-Sterile 10X · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TBS10X_500 ml 10X 500 ml
DCP-TBS10X_1000 ml 10X 1000 ml
Tris Buffered Saline TBS, 20X (Tris Buffered Saline), Non-Sterile 20X · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TBS20X_500 ml 20X 500 ml
DCP-TBS20X_1000 ml 20X 1000 ml
Tris Buffered Saline TBST (Tris-buffered saline, 0.1% Tween 20) 0.1% Tween 20 · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TBST0.1X_500ml 0.1% Tween 20 500 ml
DCP-TBST0.1X_1 L 0.1% Tween 20 1000 ml
Tris-HCl Buffer, Sterile Sterile · 3 catalog numbers · 100 ml · 500 ml · 1000 ml
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DCP-THB1X_100 ml Sterile 100 ml
DCP-THB1X_500 ml Sterile 500 ml
DCP-THB1X_1000 ml Sterile 1000 ml

Not sure which one? Check the Tris chemistry reference · See applications by format · Read the FAQ

Family Snapshot

One buffer chemistry, five ways to buy it

Tris-buffered saline (TBS) is an isotonic, non-toxic buffer used in various biochemical experiments to maintain a narrow, slightly alkaline pH range of 7-9. While the conjugate acid of Tris has a pKa of 8.07 at 25 °C, the solution pH shift is observed as temperature changes (0.03 units per degree Celsius). TBS buffer is commonly used as a laboratory reagent for washing, diluting, cell culture processing, Western blotting, ELISA and multiple assays and immunohistochemistry steps. Tris acts as a buffering agent, and NaCl regulates the solution’s tonicity, allowing it to be isotonic or hypertonic based on the desired concentration.

  • Three TBS strengths: 1X, 10X and 20X, all published as Non-Sterile.
  • One detergent format: TBST — Tris-buffered saline with 0.1% Tween 20.
  • One sterile format: Tris-HCl Buffer, Sterile, in 100 ml, 500 ml and 1000 ml.
  • Buffering chemistry: Tris acts as the buffering agent and NaCl regulates tonicity, allowing the solution to be isotonic or hypertonic based on the desired concentration.
  • pH behaviour: a narrow, slightly alkaline range of 7–9, with the conjugate acid at pKa 8.07 at 25 °C and a shift of 0.03 units per degree Celsius.
  • Contaminant control: Protease (−), DNAse (−), RNAse (−), Endonuclease (−).
  • Filtration: all reagents filtered TWICE (2X) through 0.1 micron membrane filters. No mycoplasma.
pKa 8.07 @ 25 °C 0.1 µm FILTERED 2X RUO
CATEGORY REFERENCE · TRIS-BASED BUFFERS
The published specification, in the source’s own values
  • CategoryTris-based Buffers
  • Conjugate acid pKa (25 °C)8.07
  • pH shift with temperature0.03 units per °C
  • Buffered pH range7–9
  • Buffering agentTris
  • Tonicity agentNaCl
  • TBS strengths stocked1X, 10X, 20X
  • Detergent formatTBST, 0.1% Tween 20
  • Nuclease / protease statusProtease (−), DNAse (−), RNAse (−), Endonuclease (−)
  • Filtration0.1 µm membrane, twice (2X); no mycoplasma
Why The Tris Base Matters

A mild buffer with one property you have to plan around

Tris is the default buffer of the protein bench for reasons the source states plainly — and it carries one well-documented quirk that decides whether a cold-room protocol lands where you think it does. Cards citing a bracketed number are supported by the reference list at the foot of the page.

science

Buffering where proteins live

TBS holds a narrow, slightly alkaline pH range of 7–9, with the conjugate acid of Tris at pKa 8.07 at 25 °C — the region where most protein work is done.

thermostat

pH moves with temperature

The source states the shift directly: 0.03 pH units per degree Celsius. Adjust the pH at the temperature the buffer will actually be used at, not at the bench and then in the cold room.

tune

Tonicity is a separate dial

Tris does the buffering; NaCl regulates the solution’s tonicity, allowing it to be isotonic or hypertonic based on the desired concentration. Two independent variables, two independent decisions.

handshake

Mild toward the sample

The source describes Tris-HCl as a mild buffer that does not significantly interfere with or inhibit most biochemical processes and protein-protein interactions, and whose buffering capacity prevents abrupt pH changes during purification that could denature or degrade proteins.

biotech

The buffer the standard methods were written in

Tris-glycine gel and running-buffer systems[7] and the original electrophoretic transfer and immunodetection procedure[8] are both built on Tris, which is why so many protocols specify a Tris base rather than a phosphate one.

filter_alt

Contaminant control is specified

Protease (−), DNAse (−), RNAse (−), Endonuclease (−), all reagents filtered TWICE (2X) through 0.1 micron membrane filters, and no mycoplasma — the source states all four for the family.

The number to plan around

A Tris buffer titrated at the bench does not hold that pH in a 4 °C purification or a 37 °C incubation. At 0.03 units per degree Celsius, a twenty-degree move is roughly six-tenths of a pH unit — enough to move a protein away from the window a protocol assumes. Titrate at the working temperature.

8.07
pKa of the conjugate acid of Tris at 25 °C, as published
0.03
pH units of shift per degree Celsius of temperature change
Filtration & Purity

Two passes through a 0.1 micron membrane

The source states one purity line for the whole family, and it applies to every catalog number on this page: all reagents filtered TWICE (2X) through 0.1 micron membrane filters, with no mycoplasma, and with protease, DNAse, RNAse and endonuclease all reported negative.

  1. 1

    0.1 µm Membrane filtration I

    First pass through a 0.1 micron membrane filter, removing large particulates and aggregates.

  2. 2

    0.1 µm Membrane filtration II

    Second pass through a 0.1 micron membrane filter. The source states TWICE (2X) for all reagents in this family, and reports no mycoplasma.

On sterility labelling. The three TBS strengths and the TBST item are published as Non-Sterile, while the fifth item is published as Tris-HCl Buffer, Sterile. Both descriptions are reproduced here exactly as the source states them; the filtration line above is a process statement and is not presented as a sterility claim for the items labelled Non-Sterile. For the Certificate of Analysis, the filter specification for a given lot, or a sterile fill of a TBS strength, contact support@diagnocine.com.
Tris Chemistry Reference

The published values, and what the controlling literature says

Every numeric claim the source makes about Tris was checked twice against the primary physical-chemistry literature before this page was written. The outcome of each check is stated below, so nothing has to be taken on trust.

Parameter As published by Diagnocine Controlling literature Verification outcome
Conjugate acid pKa at 25 °C 8.07 8.075 in water by emf measurement[1,2]; 8.071 ± 0.009 by potentiometry at infinite dilution[3] check_circle Confirmed twice. Source value stands; no correction made.
pH shift with temperature 0.03 units per degree Celsius Tris has one of the largest temperature coefficients among common biological buffers, established from its dissociation constant measured from 0 to 50 °C[1] check_circle Consistent with the published temperature dependence.
Working pH range 7–9, narrow and slightly alkaline Consistent with a pKa near 8.07, since useful buffering spans roughly one pH unit either side of the pKa[4] check_circle Consistent.
Buffering agent / tonicity agent Tris buffers; NaCl regulates tonicity Not a literature question — a formulation statement Reproduced as published.
Detergent in TBST 0.1% Tween 20 Tween 20 was introduced into this workflow as a non-ionic detergent that blocks unoccupied protein-binding sites on nitrocellulose[5] Reproduced as published; see the FAQ for the caveat on Tween 20 used alone[6].
One clarification beyond the source (delete if unwanted). The direction of the shift matters as much as its size: because the pKa falls as temperature rises, a Tris solution titrated to a given pH at room temperature reads higher in a 4 °C cold room and lower at 37 °C.[1] The source states the magnitude (0.03 units per degree Celsius) but not the direction; this sentence is the only thing on this page added beyond it.
Applications by Format

Which format for which bench workflow

The source publishes two application lists: one for TBS and one for Tris-HCl buffer. Each is reproduced below under the formats it belongs to, one item per line, with nothing added.

Tris Buffered Saline · 1X ready-strength, 10X and 20X concentrates · Non-Sterile
  • Washing
  • Diluting
  • Cell culture processing
  • Western blotting
  • ELISA and multiple assays
  • Immunohistochemistry steps
Tris-buffered saline with 0.1% Tween 20 · Non-Sterile
  • The same TBS application list applies, since the source publishes TBST as Tris-buffered saline carrying 0.1% Tween 20: washing, diluting, cell culture processing, Western blotting, ELISA and multiple assays, and immunohistochemistry steps.
  • The detergent is what distinguishes it: Tween 20 entered this workflow as a non-ionic detergent used to block unoccupied protein-binding sites on nitrocellulose.[5]
  • Blocking and washing choices are not interchangeable — a direct comparison of albumin, milk and Tween 20 on nitrocellulose found both quantitative and qualitative differences in the antigens detected.[9]
Tris-HCl Buffer · Sterile · 100 ml, 500 ml, 1000 ml
  • Gel electrophoresis
  • Nucleic acid work
  • Protein extraction and purification
  • Preparing SDS-PAGE gel solutions
  • Electrophoresis running buffers
  • Cell culture media
Product Comparison

The five products side by side

Every value in this table is reproduced from the catalog exactly as published, including the group label the source places over the first four rows.

Product Source group Format Catalog numbers Sizes
TBS, 1X (Tris Buffered Saline), Non-Sterile Tris Buffered Saline 1X DCP-TBS1X_500ml
DCP-TBS1X_1000ml
DCP-TBS1X_10 Liter		 500 ml, 1000 ml, 10 L
TBS, 10X (Tris Buffered Saline), Non-Sterile Tris Buffered Saline 10X DCP-TBS10X_500 ml
DCP-TBS10X_1000 ml		 500 ml, 1000 ml
TBS, 20X (Tris Buffered Saline), Non-Sterile Tris Buffered Saline 20X DCP-TBS20X_500 ml
DCP-TBS20X_1000 ml		 500 ml, 1000 ml
TBST (Tris-buffered saline, 0.1% Tween 20) Tris Buffered Saline 0.1% Tween 20 DCP-TBST0.1X_500ml
DCP-TBST0.1X_1 L		 500 ml, 1000 ml
Tris-HCl Buffer, Sterile no group label in source Sterile DCP-THB1X_100 ml
DCP-THB1X_500 ml
DCP-THB1X_1000 ml		 100 ml, 500 ml, 1000 ml
FAQ

Frequently asked questions

The questions that come up when a purchasing spec meets a bench protocol.

Tris-buffered saline is an isotonic, non-toxic buffer used in various biochemical experiments to maintain a narrow, slightly alkaline pH range of 7–9. The source lists it as a laboratory reagent for washing, diluting, cell culture processing, Western blotting, ELISA and multiple assays, and immunohistochemistry steps. Tris acts as the buffering agent and NaCl regulates the solution’s tonicity, allowing it to be isotonic or hypertonic based on the desired concentration.
Because the pKa of the conjugate acid of Tris is temperature-dependent. The source states the magnitude: 0.03 pH units per degree Celsius. Since the pKa falls as temperature rises, a solution titrated at room temperature reads higher at 4 °C and lower at 37 °C.[1] Titrate at the temperature the buffer will actually be used at.
They are the same Tris-buffered saline published at three strengths: 1X at working strength, and 10X and 20X as concentrates at ten and twenty times that strength. All three are published as Non-Sterile, and 1X is the only one offered in a 10 L fill. The source does not publish a dilution protocol or a final formulation for the concentrates — ask support@diagnocine.com if you need the composition on paper.
TBST is published as Tris-buffered saline carrying 0.1% Tween 20 — the same base with a non-ionic detergent in it. Tween 20 entered this workflow as an agent that blocks unoccupied protein-binding sites on nitrocellulose in place of a protein.[5] One caveat worth knowing: Tween 20 used alone as the blocking agent has been reported to give artefactual results, so it is generally used alongside a protein blocker rather than instead of one.[6]
It depends on the item, and the source is explicit about it. The three TBS strengths and TBST are published as Non-Sterile. The fifth item is published as Tris-HCl Buffer, Sterile. Separately, the source states that all reagents in this family are filtered TWICE (2X) through 0.1 micron membrane filters and that there is no mycoplasma. Treat the filtration line as a process statement and the item name as the sterility designation; for a Certificate of Analysis, contact support@diagnocine.com.
The source reports Protease (−), DNAse (−), RNAse (−) and Endonuclease (−) for the family, together with filtration TWICE (2X) through 0.1 micron membrane filters and no mycoplasma. That combination is what makes the family usable for nucleic acid work and protein extraction as well as for immunoassay washing steps.
Twelve catalog numbers across five product pages. TBS 1X is offered in 500 ml, 1000 ml and 10 L; TBS 10X, TBS 20X and TBST in 500 ml and 1000 ml; and Tris-HCl Buffer, Sterile in 100 ml, 500 ml and 1000 ml. The catalog table at the top of this page groups every catalog number under the product page it orders from.
Key References

Physical-chemistry and method literature behind the values above

The Diagnocine source description carries no citation list of its own. The sources below are the ones used to verify its numbers and to support the cited statements on this page; each was checked against its published record before it was listed.

  1. Bates RG, Hetzer HB (1961). Dissociation constant of the protonated acid form of 2-amino-2-(hydroxymethyl)-1,3-propanediol [tris(hydroxymethyl)aminomethane] and related thermodynamic quantities from 0 to 50°. J Phys Chem 65(4):667–671. doi:10.1021/j100822a017 — the primary determination of the Tris pKa and its temperature dependence.
  2. Etz ES, Robinson RA, Bates RG (1973). Dissociation constant of protonated tris(hydroxymethyl)aminomethane in N-methylpropionamide and related thermodynamic quantities from 10 to 55°C. J Solution Chem 2(4):405–415. doi:10.1007/BF00713253 — second, independent statement of pKa 8.075 in water at 25 °C.
  3. Schindler P, Robinson RA, Bates RG (1968). Solubility of tris(hydroxymethyl)aminomethane in water-methanol solvent mixtures and medium effects in the dissociation of the protonated base. J Res Natl Bur Stand 72A(2):141–148. doi:10.6028/jres.072A.014
  4. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM (1966). Hydrogen ion buffers for biological research. Biochemistry 5(2):467–477. doi:10.1021/bi00866a011
  5. Batteiger B, Newhall WJ 5th, Jones RB (1982). The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 55(3):297–307. doi:10.1016/0022-1759(82)90089-8
  6. Bird CR, Gearing AJH, Thorpe R (1988). The use of Tween 20 alone as a blocking agent for immunoblotting can cause artefactual results. J Immunol Methods 106(2):175–179.
  7. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259):680–685. doi:10.1038/227680a0 — the Tris-based discontinuous gel and running-buffer system.
  8. Towbin H, Staehelin T, Gordon J (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76(9):4350–4354. doi:10.1073/pnas.76.9.4350
  9. Spinola SM, Cannon JG (1985). Different blocking agents cause variation in the immunologic detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 81(1):161–165. doi:10.1016/0022-1759(85)90132-2
Format selection support. For help choosing between the 1X, 10X and 20X strengths, for the composition or dilution instructions behind a catalog item, or for documentation requests, contact support@diagnocine.com. Ready to order? Back to the Tris-based Buffers catalog.

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