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Tris Based Washing Buffers

Product#: TrisBasedWashingBuffers
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verified Sterile-Filtered · 0.1 µm ×2 + 0.04 µm ×2

Tris Based Washing Buffers - Category Selection Guide

This is a category page, not a product page — pick the wash format your protocol needs and click View to open its product page, where sizes, documentation and ordering live.

1Tris Based Washing Buffers

Five wash buffers, ten catalog numbers. They differ by detergent, by detergent percentage, and by concentration factor.

Tris Based Washing Buffers — Catalog · 10 Catalog Numbers Across 5 Product Pages
Catalog numbers are grouped under the product page they order from; click View once per group.
Cat. No. Size
Washing Buffer Tris NP-40 Buffer NP-40 · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TNP1X_500 ml 500 ml
DCP-TNP1X_1000 ml 1000 ml
Washing Buffer TBST (Tris-buffered saline, 0.1% Tween 20) [1X] 0.1% Tween 20 · 1X · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TBST0.1X_500ml 500 ml
DCP-TBST0.1X_1000ml 1000 ml
Washing Buffer Tris-Buffered Saline (TBS) with (0.05%) Tween-20 [1X] 0.05% Tween-20 · 1X · 2 catalog numbers · 100 ml · 500 ml
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DCP-TBST1X_100 ml 100 ml
DCP-TBST1X_500 ml 500 ml
Washing Buffer TBST (Tris-buffered saline, 0.05% Tween 20) [20X] 0.05% Tween 20 · 20X concentrate · 2 catalog numbers · 500 ml · 1000 ml
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DCP-TBST20X_500 ml 500 ml
DCP-TBST20X_1000 ml 1000 ml
Washing Buffer Tris Triton-X Buffer Triton-X · 2 catalog numbers · 100 ml · 500 ml
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DCP-TTXB1X_100 ml 100 ml
DCP-TTXB1X_500 ml 500 ml

2Protease Inhibitor Cocktail with Tris Based Washing Buffers

Three ready-to-use combinations, three catalog numbers. Each pairs a seven-component protease inhibitor cocktail with one of the washing buffers above.

Protease Inhibitor Cocktail with Tris Based Washing Buffers — Catalog · 3 Catalog Numbers Across 3 Product Pages
One catalog number per product page; click View once per group.
Cat. No. Size
Protease Inhibitor Cocktail Protease Inhibitor Cocktail with NP-40 Buffer NP-40 · Ready-to-use · 1 catalog number · 10 x 10 mL
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DCP-PICTNP1X 10 x 10 mL
Protease Inhibitor Cocktail Protease Inhibitor Cocktail with Tris Triton-X Buffer Triton-X · Ready-to-use · 1 catalog number · 10 x 10 mL
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DCP-PICTTX1X 10 x 10 mL
Protease Inhibitor Cocktail Protease Inhibitor Cocktail with Tris Tween 20 Buffer Tween 20 · Ready-to-use · 1 catalog number · 10 x 10 mL
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DCP-PICTT201X 10 x 10 mL

Not sure which format? Browse the washing-buffer formats · See the cocktail components · Read the FAQ

Part 1 · Tris Based Washing Buffers

What a Tris based washing buffer is for

Tris-based washing buffers are essential solutions used in various biochemical and molecular biology applications. They help remove unbound materials and reduce background noise, improving assay sensitivity and specificity. These buffers are primarily composed of Tris (tris(hydroxymethyl)aminomethane), which is a widely used biological buffer due to its effective pH buffering capacity in the range of 7.0 to 8.0.

Five product pages, ten catalog numbers. What separates them is the detergent, the detergent percentage, and the concentration factor. The three protease-inhibited combinations are a separate family and are described in Part 2.

  • Five products, ten catalog numbers: Tris NP-40 Buffer, TBST (0.1% Tween 20) [1X], Tris-Buffered Saline (TBS) with (0.05%) Tween-20 [1X], TBST (0.05% Tween 20) [20X], and Tris Triton-X Buffer.
  • One base chemistry: every product in this group is built on Tris (tris(hydroxymethyl)aminomethane), stated by the source as buffering effectively across pH 7.0 to 8.0.
  • Three detergent formats: NP-40, Tween 20, and Triton-X, as published in the product names.
  • Two Tween 20 percentages: 0.1% and 0.05% — the source lists both as separate products, not as a single configurable item.
  • Ready-to-use and concentrate: four products at 1X plus one 20X concentrate (TBST, 0.05% Tween 20).
  • Fill sizes: 100 ml, 500 ml, and 1000 ml.
  • Sterile filtration: filtered through a 0.1 micron membrane twice and a 0.04 micron membrane twice, in a sterile environment; stored at 4 °C and shipped at room temperature.
  • Customization: other concentrations, added chemicals, compounds, proteins or supplements, different pH, and other modifications are available on inquiry.
Sterile-Filtered Customizable RUO
PART 1 · TRIS BASED WASHING BUFFERS
The washing buffers at a glance — chemistry, formats, and what actually differs between the five product pages
  • Base bufferTris
  • Stated buffering rangepH 7.0 to 8.0
  • Products in this group5
  • Catalog numbers10
  • Detergent formatsNP-40 / Tween 20 / Triton-X
  • Tween 20 percentages0.1% and 0.05%
  • Concentrations offered1X and 20X
  • Fill sizes100 / 500 / 1000 ml
  • Sterile filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage / shipping4 °C / room temperature
On the stated pH 7.0 to 8.0 range. The source describes Tris as buffering effectively across pH 7.0 to 8.0, which is correct and is the window most wash protocols sit in. For context beyond the source: Tris has a pKa near 8.06 at 25 °C, and vendor documentation commonly states a working range of pH 7.0 to 9.0. Tris also has an unusually large temperature coefficient — roughly −0.028 pH units per °C — so a buffer titrated at room temperature reads higher in a 4 °C cold room and lower at 37 °C. Set and verify pH at the temperature the wash will actually run at, and consult the Certificate of Analysis for the pH of the lot you receive.
Why the Wash Format Matters

A wash step is where signal is won or lost

Every product on this page does the same job — carry unbound material away without disturbing what is meant to stay bound. The differences below are the ones the source itself draws, and they are the ones that decide which bottle belongs on your bench.

water_drop

Removing unbound material

The stated purpose of the family: help remove unbound materials so that only specifically bound species remain to be detected. Everything else about a wash buffer is in service of that one job.

visibility

Reducing background noise

The source names background reduction as the second function, with the direct consequence that assay sensitivity and specificity improve. A dirty background costs you the low end of your dynamic range.

science

Tris as the base

All eight product pages share one buffering chemistry: Tris, tris(hydroxymethyl)aminomethane, described by the source as a widely used biological buffer effective across pH 7.0 to 8.0.

tune

Detergent and percentage

The family is published as NP-40, Tween 20, and Triton-X formats, with Tween 20 offered at two percentages — 0.1% and 0.05%. The source lists these as distinct products; match the one your protocol names.

shield

Preserving protein integrity

The protease inhibitor cocktail formats of Part 2 are described as a ready-to-use combination that removes unbound material and reduces background noise while preserving protein integrity — the reason to reach for them rather than a plain wash buffer.

edit_note

When the catalog does not fit

Other concentrations, additions of chemicals, compounds, proteins or supplements, a different pH, and other modifications are available on inquiry. Ask before you compromise on a formulation.

Three questions decide which of the eight pages you want

Which detergent does your protocol name — NP-40, Tween 20, or Triton-X? If Tween 20, at what percentage — 0.1% or 0.05%? And do you need the protease inhibitor cocktail already in the bottle, which moves you from Part 1 to Part 2? Answer those three and the catalog above narrows to one group.

10
catalog numbers in Part 1, across 5 product pages
3
ready-to-use cocktail formats in Part 2
Part 1 · Filtration Architecture

Four membrane passes before the bottle is filled

The source states one sterility process for the Tris Based Washing Buffers: filtration through a 0.1 micron membrane twice and a 0.04 micron membrane twice, carried out in a sterile environment. The four stages below are that process, in order.

  1. 1

    0.1 µm Pre-filtration I

    The first 0.1 micron pass takes out the bulk particulate load before any finer membrane sees the fluid, so the downstream stages are not asked to do coarse work.

  2. 2

    0.04 µm Pre-filtration II

    A 0.04 micron pass follows, reaching well below the 0.1 micron cut and removing sub-micron material that a coarser membrane leaves in solution.

  3. 3

    0.1 µm Sterile-filtration I

    The second 0.1 micron pass is the sterile-filtration stage, performed in the sterile environment the source specifies for the fill.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    The final 0.04 micron pass is the polishing stage: the last thing the buffer passes through before it reaches the bottle you open at the bench.

Why a wash buffer is worth filtering this hard

A wash buffer touches the assay surface more times than any other reagent in the workflow, and it touches it last. Particulate carried in by the wash is particulate deposited on the membrane, the well, or the channel — which is exactly the background the buffer is there to remove.

4
membrane passes stated by the source
0.04
micron rating of the final polishing pass
Scope of the handling specifications. Storage at 4 °C, shipping at room temperature, and the filtration process above are published by the source for the Tris Based Washing Buffers group. For the three protease inhibitor cocktail formats the product page is the controlling record — open it from the catalog above, or contact support@diagnocine.com for the current documentation.
Quadruple-stage filtration diagram for Diagnocine Tris Based Washing Buffers: 0.1 micron pre-filtration, 0.04 micron pre-filtration, 0.1 micron sterile filtration, and a final 0.04 micron sub-0.1 micron polishing pass, filled in a sterile environment - the process published for the TBST, Tris NP-40 and Tris Triton-X wash buffer family
Figure 1. Quadruple-stage filtration — two 0.1 micron passes and two 0.04 micron passes, the last of them a final polish before the sterile fill. © Diagnocine® — Tris Based Washing Buffers
Part 2 · Protease-Inhibited Formats

Protease Inhibitor Cocktail with Tris Based Washing Buffers

These Ready-to-use combination of a protease inhibitor cocktail with various washing buffers provides a powerful tool for removing unbound materials and reducing background noise while preserving protein integrity. The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor.

  • Protease Inhibitor Cocktail with NP-40 Buffer — DCP-PICTNP1X · 10 x 10 mL
  • Protease Inhibitor Cocktail with Tris Triton-X Buffer — DCP-PICTTX1X · 10 x 10 mL
  • Protease Inhibitor Cocktail with Tris Tween 20 Buffer — DCP-PICTT201X · 10 x 10 mL
  • All three are supplied ready to use; the washing buffer and the seven-component cocktail arrive combined in one bottle.

The seven cocktail components

Component Role, as published by the source
AEBSF.HCl A serine protease inhibitor
Aprotinin Targets serine proteases and has anti-inflammatory properties
Bestatin An aminopeptidase inhibitor[6]
E-64 A cysteine protease inhibitor[5]
EDTA A metalloproteinase inhibitor
Leupeptin Inhibits both serine and cysteine proteases
Pepstatin A An aspartic protease inhibitor[7]
Part 1 · Formats

The washing buffers, grouped by detergent

Select a detergent to see the products the source lists under it. The source describes one shared function for the whole group; what changes between tabs is the detergent, the percentage, and the concentration factor.

Tween 20 · 0.1% and 0.05% · 1X and 20X
  • TBST (Tris-buffered saline, 0.1% Tween 20) [1X] — 500 ml and 1000 ml
  • Tris-Buffered Saline (TBS) with (0.05%) Tween-20 [1X] — 100 ml and 500 ml
  • TBST (Tris-buffered saline, 0.05% Tween 20) [20X] — 500 ml and 1000 ml
  • Shared function: help remove unbound materials and reduce background noise, improving assay sensitivity and specificity
  • The 20X entry is the only concentrate in this group; the other two are supplied at 1X
NP-40 · Triton-X · 1X
  • Tris NP-40 Buffer — 500 ml and 1000 ml
  • Tris Triton-X Buffer — 100 ml and 500 ml
  • Shared function: help remove unbound materials and reduce background noise, improving assay sensitivity and specificity
  • Both are built on the same Tris base as the Tween 20 formats, with a different detergent
Shared Specifications

What every product on this page has in common

Values reproduced from the source. Where a specification is published for only one of the two families, the row is badged Part 1 (Tris Based Washing Buffers) or Part 2 (Protease Inhibitor Cocktail formats).

Specification As published
Base buffer Tris (tris(hydroxymethyl)aminomethane)
Stated buffering range pH 7.0 to 8.0
Storage Part 1 4 °C
Shipping Part 1 Room temperature
Sterility Part 1 Filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment
Detergents offered NP-40, Tween 20 (0.1% and 0.05%), Triton-X
Concentrations offered 1X and 20X
Fill sizes 100 ml, 500 ml, 1000 ml (Part 1); 10 x 10 mL (Part 2)
Cocktail composition Part 2 AEBSF.HCl, Aprotinin, Bestatin, E-64, EDTA, Leupeptin, Pepstatin A
Customization Other concentrations, added chemicals / compounds / proteins / supplements, different pH, and other modifications on inquiry
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed. Write to support@diagnocine.com.
FAQ

Frequently asked questions

The questions that come up most often when a protocol calls for a Tris based wash and eight product pages are on the screen.

They are used across biochemical and molecular biology applications to help remove unbound materials and reduce background noise, which improves assay sensitivity and specificity. The buffering chemistry is Tris — tris(hydroxymethyl)aminomethane — described by the source as effective across pH 7.0 to 8.0.
They are listed as separate products, not as one configurable item. TBST (Tris-buffered saline, 0.1% Tween 20) [1X] is supplied in 500 ml and 1000 ml. Tris-Buffered Saline (TBS) with (0.05%) Tween-20 [1X] is supplied in 100 ml and 500 ml. A third product, TBST (Tris-buffered saline, 0.05% Tween 20) [20X], carries the same 0.05% detergent level as a 20X concentrate. Match whichever percentage your published protocol names.
One product in the family is listed at 20X: TBST (Tris-buffered saline, 0.05% Tween 20) [20X], catalog numbers DCP-TBST20X_500 ml and DCP-TBST20X_1000 ml. Every other washing buffer on this page is listed at 1X.
Seven components. AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor.
Through a 0.1 micron membrane twice and a 0.04 micron membrane twice, in a sterile environment. That is four membrane passes, the last of them a 0.04 micron polishing stage before the fill.
The source specifies storage at 4 °C and shipping at room temperature for the Tris Based Washing Buffers. For the three protease inhibitor cocktail formats, the product page is the controlling record — open it from the catalog, or write to support@diagnocine.com.
Both statements are consistent. Tris has a pKa near 8.06 at 25 °C, and a buffer works roughly one pH unit either side of its pKa, so the published working range is commonly given as pH 7.0 to 9.0; pH 7.0 to 8.0 is the part of that range wash protocols actually sit in. Worth knowing at the bench: Tris has a large temperature coefficient, about −0.028 pH units per °C, so a buffer set at room temperature reads higher in a 4 °C cold room and lower at 37 °C. Set and check pH at the temperature the wash will run at. This answer goes beyond the source description and can be removed.
Key References

Supporting literature

The source description carries no citation list, so the curated primary papers below are supplied as supporting literature and were each checked against the primary record before being written. They cover buffer chemistry and the Tris comparison (1); protein blotting to nitrocellulose (2); the use of Tween 20 as a blocking agent on nitrocellulose (3); the original ELISA (4); and the first characterizations of E-64, bestatin and pepstatin (5–8). They describe the methods, not these products.

  1. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM (1966). Hydrogen ion buffers for biological research. Biochemistry 5(2):467–477. doi:10.1021/bi00866a011
  2. Towbin H, Staehelin T, Gordon J (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76(9):4350–4354. doi:10.1073/pnas.76.9.4350
  3. Batteiger B, Newhall WJ, Jones RB (1982). The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. J Immunol Methods 55(3):297–307. doi:10.1016/0022-1759(82)90089-8
  4. Engvall E, Perlmann P (1971). Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8(9):871–874. doi:10.1016/0019-2791(71)90454-X
  5. Barrett AJ, Kembhavi AA, Brown MA, Kirschke H, Knight CG, Tamai M, Hanada K (1982). L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J 201(1):189–198. doi:10.1042/bj2010189
  6. Umezawa H, Aoyagi T, Suda H, Hamada M, Takeuchi T (1976). Bestatin, an inhibitor of aminopeptidase B, produced by actinomycetes. J Antibiot (Tokyo) 29(1):97–99. doi:10.7164/antibiotics.29.97
  7. Umezawa H, Aoyagi T, Morishima H, Matsuzaki M, Hamada M (1970). Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo) 23(5):259–262. doi:10.7164/antibiotics.23.259
  8. Suda H, Aoyagi T, Takeuchi T, Umezawa H (1976). Inhibition of aminopeptidase B and leucine aminopeptidase by bestatin and its stereoisomer. Arch Biochem Biophys 177(1):196–200. doi:10.1016/0003-9861(76)90429-X
Format selection support. For help matching a wash format to a specific protocol, or for documentation requests, contact support@diagnocine.com. Ready to order? Back to the Tris Based Washing Buffers catalog.

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