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Tris Based Running Buffers

Product#: TrisBasedRunningBuffers
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verified Quadruple-Stage Filtered · Sterile-Environment Fill

Tris Based Running Buffers - Category Selection Guide

This is a category page, not a product page. Pick the buffer your gel calls for and click View to open its product page — the shared specification, the filtration architecture, and the application guidance follow below.

Tris Based Running Buffers — Catalog · 14 Catalog Rows Across 7 Product Pages
This page is not a product page. Catalog numbers are grouped under the product page they order from — click View once per group.
Cat. No. pH Size
Tris-Tricine SDS Running Buffer pH 8.3 · DCP-TTSDSRB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-TTSDSRB1X 8.3 500 ml
DCP-TTSDSRB1X 8.3 1000 ml
Tris-Glycine SDS Buffer pH 8.3 · DCP-TGSB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-TGSB1X 8.3 500 ml
DCP-TGSB1X 8.3 1000 ml
Tris-Glycine Non-Denaturing Buffer pH 8.3 · DCP-TGNDB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-TGNDB1X 8.3 500 ml
DCP-TGNDB1X 8.3 1000 ml
MES SDS Running Buffer pH 7.3 · DCP-MESSDSRB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-MESSDSRB1X 7.3 500 ml
DCP-MESSDSRB1X 7.3 1000 ml
MOPS SDS Running Buffer pH 7.7 · DCP-MOPSSDSRB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-MOPSSDSRB1X 7.7 500 ml
DCP-MOPSSDSRB1X 7.7 1000 ml
Tris Acetate EDTA Buffer pH 8.0 · DCP-TAE1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-TAE1X 8.0 500 ml
DCP-TAE1X 8.0 1000 ml
Tris Borate EDTA Running Buffer pH 8.3 · DCP-TBERB1X · 2 fill sizes · 500 ml · 1000 ml
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DCP-TBERB1X 8.3 500 ml
DCP-TBERB1X 8.3 1000 ml

Not sure which buffer? See the shared specification · See the buffers by group · Read the FAQ

Category Snapshot

What the tank buffer is actually doing

Tris based buffers play a crucial role in maintaining consistent pH, providing ions for conductivity, and ensuring efficient and reproducible protein separation in gel electrophoresis experiments. Tris provides effective buffering in the alkaline range.

The specification every buffer in this family shares, and the one value that separates them.

  • Seven buffers stocked: Tris-Tricine SDS Running Buffer, Tris-Glycine SDS Buffer, Tris-Glycine Non-Denaturing Buffer, MES SDS Running Buffer, MOPS SDS Running Buffer, Tris Acetate EDTA Buffer, and Tris Borate EDTA Running Buffer.
  • Three jobs, one solution: consistent pH, ions for conductivity, and efficient, reproducible protein separation.
  • pH is the differentiator: the family runs from 7.3 to 8.3 — MES at 7.3, MOPS at 7.7, Tris Acetate EDTA at 8.0, and the remaining four at 8.3.
  • Alkaline-range buffering: Tris provides effective buffering in the alkaline range.
  • Quadruple-stage filtration: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment — sub-0.1 µm polishing on every fill.
  • Appearance: clear, colorless liquid — a one-second incoming check before a bottle goes on the bench.
  • Two fill volumes: every product is offered in 500 ml and 1000 ml under one catalog number.
Quadruple-Stage Sterile Environment RUO
CATEGORY REFERENCE · TRIS BASED RUNNING BUFFERS
Buffer selection at a glance — pH, fill volume, and the specification shared across the family
  • Products in this category7
  • Catalog rows (product × fill size)14
  • Fill volumes500 ml · 1000 ml
  • Stated pH values7.3 · 7.7 · 8.0 · 8.3
  • Lowest stated pH7.3 (MES SDS Running Buffer)
  • Highest stated pH8.3 (four products)
  • Filtration architecture0.1 µm ×2 + 0.04 µm ×2
  • Fill environmentsterile environment
  • Appearanceclear, colorless liquid
  • Tris buffering rangealkaline
Why the Running Buffer Matters

Three jobs the tank buffer has to do at once

A running buffer is not just the liquid the gel sits in. Each panel below is one of the roles the source assigns to it, plus the two specifications that decide whether a given lot can play them.

speed

Consistent pH

Tris based buffers play a crucial role in maintaining consistent pH through the run. Every product in this family carries its own stated pH, from 7.3 to 8.3.

bolt

Ions for conductivity

The buffer provides the ions that carry current through the gel. Without a defined ionic supply there is no reproducible field, and without that there is no reproducible migration.

rule

Efficient, reproducible separation

The point of the first two jobs is the third: ensuring efficient and reproducible protein separation in gel electrophoresis experiments.

science

Buffering in the alkaline range

Tris provides effective buffering in the alkaline range, which is where four of the seven products in this family sit, at pH 8.3.

filter_alt

Ultra-low particulate by architecture

Filtered 0.1 micron twice and 0.04 micron twice in a sterile environment. Sub-0.1 µm polishing is what makes these buffers microchannel-safe for automated and microfluidic handling.

visibility

Clear, colorless liquid

Appearance is a specification, not a description. A clear, colorless liquid is the expected state on receipt, and any deviation is worth raising before the bottle is poured into a tank.

Read the pH, then read the name

Every product in this family shares the same filtration architecture, the same sterile-environment fill, the same appearance specification, and the same two fill volumes. The one specification the source varies across the catalog is pH — 7.3, 7.7, 8.0, or 8.3 — so that is the value to match against the protocol before anything else.

7
products, each offered in 500 ml and 1000 ml under one catalog number
7.3–8.3
the full span of stated pH across the family
Filtration Architecture

Quadruple-stage: 0.1 µm twice, 0.04 µm twice

The source states one sterility specification for the whole family: filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment. Four membrane passes, in this order.

  1. 1

    0.1 µm Pre-filtration I

    The first 0.1 micron pass takes out the bulk particulate load before any polishing membrane sees the solution.

  2. 2

    0.04 µm Pre-filtration II

    The first 0.04 micron pass drops the retained size well below what a conventional sterilizing filter reaches.

  3. 3

    0.1 µm Sterile-filtration I

    The second 0.1 micron pass is run inside the sterile environment in which the product is filled.

  4. 4

    0.04 µm Sterile-filtration II — Final Polish

    The final 0.04 micron pass is the last thing the solution passes through before it reaches the bottle.

Why the last pass is the one that matters

A 0.04 micron final polish is the specification that lets these buffers be described as ultra-low particulate and microchannel-safe. It is the architecture, not an adjective, that supports the claim — and it is stated identically for every product in this family.

4
membrane passes per lot — two at 0.1 µm, two at 0.04 µm
0.04
µm — the final polishing membrane before fill
Filtration is a per-lot process specification. Filtration method and pass count are set per order and can change; the architecture described here is the one the current source states for this family. For a Certificate of Analysis or a written process statement for a specific lot, contact support@diagnocine.com.
Quadruple-stage filtration diagram for Diagnocine Tris Based Running Buffers, showing four membrane passes: 0.1 micron pre-filtration, 0.04 micron pre-filtration, 0.1 micron sterile filtration, and a final 0.04 micron sterile-filtration polish before sterile-environment fill of Tris-Tricine, Tris-Glycine, MES, MOPS, Tris Acetate EDTA and Tris Borate EDTA electrophoresis running buffers.
Figure 1. The four-pass filtration train stated for this family — 0.1 µm twice and 0.04 µm twice, ending in a 0.04 µm final polish before sterile-environment fill. © Diagnocine® — Tris Based Running Buffers
Shared Specifications

What every buffer in this family has in common

Four of the five specifications below are identical across all seven products. Only pH varies — which is why the catalog above sorts on it.

Parameter Stated specification Scope
Sterility Filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment All 7 products
Appearance Clear, colorless liquid All 7 products
Fill volumes 500 ml and 1000 ml All 7 products
Buffering range (Tris) Effective buffering in the alkaline range Stated for the category
pH 7.3, 7.7, 8.0, or 8.3 depending on the product Varies — see the catalog
Customization. pH, molarity / concentration, ionic strength, salt composition, and additive content are available on request — contact support@diagnocine.com.
The Family by Group

How the seven buffers group by name

Select a group to see which products carry that token in their source name, and what the source states about the family's function.

Four products · pH 7.3 to 8.3
  • Tris-Tricine SDS Running Buffer — pH 8.3
  • Tris-Glycine SDS Buffer — pH 8.3
  • MES SDS Running Buffer — pH 7.3
  • MOPS SDS Running Buffer — pH 7.7
  • All four maintain consistent pH, provide ions for conductivity, and support efficient and reproducible protein separation in gel electrophoresis experiments.
One product · pH 8.3
  • Tris-Glycine Non-Denaturing Buffer — pH 8.3
  • The only product in the family whose source name states a non-denaturing format.
  • It carries the same sterility, appearance, and fill-volume specification as every other member.
Two products · pH 8.0 and 8.3
  • Tris Acetate EDTA Buffer — pH 8.0
  • Tris Borate EDTA Running Buffer — pH 8.3
  • Both carry the EDTA token in their source name and the family's shared sterility, appearance, and fill-volume specification. The source states no application for either individually — see the product page.
FAQ

Frequently asked questions

The questions that come up most often when a gel protocol meets a purchasing spec.

Three things at once. Tris based buffers play a crucial role in maintaining consistent pH, providing ions for conductivity, and ensuring efficient and reproducible protein separation in gel electrophoresis experiments. Tris provides effective buffering in the alkaline range.
MES SDS Running Buffer is pH 7.3 and MOPS SDS Running Buffer is pH 7.7. Tris Acetate EDTA Buffer is pH 8.0. Tris-Tricine SDS Running Buffer, Tris-Glycine SDS Buffer, Tris-Glycine Non-Denaturing Buffer, and Tris Borate EDTA Running Buffer are all pH 8.3.
Every product in this family is offered in 500 ml and 1000 ml, and both fill volumes sit under a single catalog number per product — fourteen catalog rows across seven catalog numbers and seven product pages. Choose the fill volume on the product page the View button opens.
Filtered 0.1 micron twice and 0.04 micron twice, in a sterile environment. That is four membrane passes per lot, ending in a 0.04 micron final polish before fill, and it is stated identically for every product in this family.
A clear, colorless liquid. Appearance is part of the stated specification, so it doubles as a one-second incoming check — anything cloudy, tinted, or carrying visible particulate is worth raising before the bottle goes into a tank.
Both are listed in this category by the source, and both are the lower-pH members of it — MES SDS Running Buffer at pH 7.3 and MOPS SDS Running Buffer at pH 7.7, against pH 8.0 and 8.3 for the rest. The source states no composition for any product in the family, so match on the stated pH and the product name your protocol calls for, and consult the product page for the formulation.
pH, molarity / concentration, ionic strength, salt composition, and additive content are available on request. Contact support@diagnocine.com with the specification your protocol needs.
Supporting Literature

The buffer systems behind these products

The source carries no citations of its own. The papers below are the primary literature defining the discontinuous and Tricine buffer systems this family is named for, each verified against the publisher record.

  1. Ornstein, L. (1964). Disc electrophoresis — I: Background and theory. Annals of the New York Academy of Sciences, 121(2), 321–349. — The theory of the discontinuous (stacking / resolving) buffer system on which Tris-based gel electrophoresis rests. doi:10.1111/j.1749-6632.1964.tb14207.x
  2. Davis, B. J. (1964). Disc electrophoresis — II: Method and application to human serum proteins. Annals of the New York Academy of Sciences, 121(2), 404–427. — The companion method paper that put the discontinuous system into practice.
  3. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680–685. — The paper that introduced the Tris-glycine SDS discontinuous system still used for routine SDS-PAGE. doi:10.1038/227680a0
  4. Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. M. (1966). Hydrogen ion buffers for biological research. Biochemistry, 5(2), 467–477. — The paper that established the design criteria for near-neutral biological buffers, MES among them. doi:10.1021/bi00866a011
  5. Schägger, H., & von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Analytical Biochemistry, 166(2), 368–379. — The original Tricine-SDS system, using Tricine as the trailing ion to resolve small proteins at lower acrylamide concentrations than glycine-SDS systems. doi:10.1016/0003-2697(87)90587-2
  6. Schägger, H. (2006). Tricine–SDS-PAGE. Nature Protocols, 1(1), 16–22. — The modern step-by-step protocol for the Tricine system, including staining and electroblotting. doi:10.1038/nprot.2006.4
Buffer selection support. For help matching a running buffer to a specific gel chemistry or protocol, or for documentation requests, contact support@diagnocine.com. Ready to order? Back to the Tris Based Running Buffers catalog.

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