Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
Sodium acetate buffer is used to maintain the ionic strength of the solution, facilitating the precipitation of DNA/RNA with alcohol. It helps to control the pH and salt composition of the solution, promoting slow crystal formation of the target protein. The glacial acetic acid provides the weak acid (acetic acid) that, along with the acetate ion, forms the buffer system. The sodium acetate buffer resists changes in pH upon adding small amounts of acid or base by utilizing the buffering action of the acetic acid/acetate ion system.
Advantages:
Provides good ionic strength in the acidic pH range.
Sodium acetate is a safe, non-toxic compound.
Suitable for applications that require a mildly acidic environment.
Applications:
It is used to create low-pH conditions for protein crystallization experiments.
Staining gels for protein electrophoresis.
Purification and precipitation of nucleic acids (DNA/RNA)
Composition:
3M Sodium Acetate
Glacial acetic acid to adjust the pH
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 5.2
Sterility: Filtered 0.1 micron Once and 0.04 micron Once in a non-sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: Room temperature
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
