Recombinant Anti-beta Actin antibody (Rabbit mAb)
Recombinant Anti-beta Actin antibody (Rabbit mAb)
General information
| Cat. No. | ：SB-GB15003-100 |
| Size | ：100 uL |
| Protein full name | ：Actin, cytoplasmic 1 |
| Synonym | ：ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta CytoskeletalActin, β-Actin |
| Immunogen | ：KLH conjugated synthetic peptidecorresponding to Mouse beta-Actin |
| Isotype | ：IgG1 |
| Purity | ：Affinity purification |
| Subcellular location | ：Cytoplasm |
| Predicted MW. | ：42 kDa |
| Observed MW. | ：45 kDa |
| Uniprot ID | ：P60710 |
| Storage | ：Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Application
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 |
HeLa, Jurkat, MCF-7 |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, colon cancer, lung, lung cancer |
| ICC/IF | Human, Mouse, Rat | 1: 300-1: 1000 | NIH/3T3, A549, etc. |
Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
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Western blot analysis of beta-Actin (GB15003) at dilution of 1: 2000 |
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Immunohistochemistry of paraffin embedded mouse spleen using beta-Actin (GB15003) at dilution of 1: 1000 |
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Immunohistochemistry of paraffin embedded human colon cancer using beta-Actin (GB15003) at dilution of 1: 1000
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Immunohistochemistry of paraffin embedded human lung cancer using beta-Actin (GB15003) at dilution of 1: 1000
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Immunohistochemistry of paraffin embedded rat lung using beta-Actin (GB15003) at dilution of 1: 1000
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Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15003) at dilution of 1: 1000
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Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using β-Actin (GB15003) at dilution of 1: 1000
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Aliases for ACTB Gene
