:KLH conjugated synthetic peptidecorresponding to Mouse beta-Actin
Isotype
:IgG1
Purity
:Affinity purification
Subcellular location
:Cytoplasm
Predicted MW.
:42 kDa
Observed MW.
:45 kDa
Uniprot ID
:P60710
Storage
:Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Application
Applications
Species
Dilution
Positive Tissue
WB
Human, Mouse, Rat
1: 1000-1: 2000
lung, brain
IHC/IF
Human, Mouse, Rat
1: 500-1: 1000
rectum, liver, colon, stomach
ICC/IF
Human, Mouse, Rat
1: 300-1: 1000
NIH/3T3, A549, etc.
Description
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Western blot analysis of β-Actin(GB15001) at dilution of 1: 1000
Western blot analysis of β-Actin(GB15001) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed mouse rectum using β-Actin (GB15001) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat small intestine using β-Actin (GB15001) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed human colon using β-Actin (GB15001) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed NIH/3T3 cells using β-Actin (GB15001) at dilution of 1: 1000
Immunocytochemistry analysis of 4% paraformaldehyde fixed A549 cells using β-Actin (GB15001) at dilution of 1: 1000