Recombinant Anti- GAPDH antibody ( Rabbit mAb )
Recombinant Anti- GAPDH antibody ( Rabbit mAb )
General information
| Cat. No. | ：SB-GB15004-100 |
| Size | ：100 uL |
| Protein full name | ：Glyceraldehyde-3-phosphate dehydrogenase |
| Synonym | ：GAPDH , GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase |
| Immunogen | ：KLH conjugated synthetic peptide corresponding to Mouse GAPDH |
| Isotype | ：IgG1 |
| Purity | ：Affinity purification |
| Subcellular location | ：Cytoplasm, Nucleus, Membrane |
| Predicted MW. | ：36 kDa |
| Observed MW. | ：36 kDa |
| Uniprot ID | ：P16858 |
| Storage | ：Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
Application
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 1000-1: 2000 |
lung, brain, etc. |
| IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, testis, etc. |
| ICC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | HeLa |
Description
Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.
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Western blot analysis of GAPDH (GB15004) at dilution of 1: 2000 |
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Immunohistochemistry of paraffin embedded mouse kidney using GAPDH (GB15004) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse testis using GAPDH (GB15004) at dilution of 1: 500
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Immunocytochemistry analysis of 4% paraformaldehyde fixed HeLa cells using GAPDH (GB15004) at dilution of 1: 500
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Aliases for GAPDH Gene
- GeneCards Symbol: GAPDH 2
- Glyceraldehyde-3-Phosphate Dehydrogenase 2 3 4 5
- GAPD 3 4 5
- Peptidyl-Cysteine S-Nitrosylase GAPDH 3 4
- EC 1.2.1.12 4 48
- Epididymis Secretory Sperm Binding Protein Li 162eP 3
- Oct1 Coactivator In S Phase, 38 Kd Component 3
- Aging-Associated Gene 9 Protein 3
- OCAS, P38 Component 3
- HEL-S-162eP 3
- EC 2.6.99.- 4
- EC 1.2.1 48
- G3PD 3