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Phosphate Based Lysis/Extraction Buffers

Phosphate-based lysis/extraction buffers provide an effective option for protein isolation, particularly when physiological conditions need to be maintained or when working with phosphate-binding proteins. Their ability to maintain stable pH and compatibility with various additives make them a valuable tool in biochemical research. 

Appearance: Clear, Colorless Liquid.
pH: 7.4
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
Storage: 4 ℃ 
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Protease Inhibitor Cocktail with Phosphate Based Lysis/Extraction Buffers

These Ready-to-use combination of a protease inhibitor cocktail with various washing buffers provides a powerful tool for efficient protein extraction while preserving protein integrity. This method utilizes detergents to disrupt cell membranes and solubilize proteins, while the carefully formulated protease inhibitor cocktail prevents protein degradation during the lysis process. The cocktail contains AEBSF.HCl, a serine protease inhibitor; Aprotinin, which targets serine proteases and has anti-inflammatory properties; Bestatin, an aminopeptidase inhibitor; E-64, a cysteine protease inhibitor; EDTA, a metalloproteinase inhibitor; Leupeptin, which inhibits both serine and cysteine proteases; and Pepstatin A, an aspartic protease inhibitor.