FluxMPS™ PBS, 1X (Phosphate Buffered Saline), Sterile — MPS-Grade Ultra-Filtered

FluxMPS™ PBS, 1X (Phosphate Buffered Saline), Sterile — MPS-Grade Ultra-Filtered

FluxMPS™ PBS 1X is an MPS-grade, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) phosphate buffered saline engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and precision microfluidic cell culture. Free of calcium, magnesium, proteases, DNase, RNase, and endonucleases, this ultra-low particulate formulation is approximately 5× cleaner than conventional 0.22 µm-filtered saline by particulate count — ensuring microchannel-safe isotonic buffering for demanding tissue-chip and lab-on-a-chip applications.

• Quadruple-stage filtration: 0.1 µm (pre-filter I) → 0.04 µm (pre-filter II) → 0.1 µm (sterile I) → 0.04 µm (final polish) — sub-mycoplasma polishing
• Endotoxin tested: < 0.05 EU/mL (USP <85> BET method), microchannel-safe
• Ca²⁺-free & Mg²⁺-free formulation — ideal for enzymatic cell dissociation & rinse workflows
• Protease (-) · DNase (-) · RNase (-) · Endonuclease (-) — nucleic-acid and protein integrity preserved
• ASTM Type 1 Ultrapure water (0.05 µS/cm, 18.2 MΩ·cm) as solvent base
• ISO Class 5 (Class 100) aseptic fill & finish in ISO 13485:2016-certified facility
• pH 7.4 ± 0.1 (25 °C) | Osmolality: 270–290 mOsm/kg
• pH, concentration (2X, 4X, 5X, 10X), and chemical/detergent/inhibitor/protein additive customization available — contact support@diagnocine.com

Engineered where standard PBS fails

Conventional 0.22 µm-filtered PBS harbors subvisible particulates (USP <788> ≥10 µm), residual mycoplasma-scale debris, and undeclared endotoxin burden that silently corrupt chip-based assays, clog microfluidic channels, and confound biosensor readouts. FluxMPS™ PBS 1X eliminates these failure modes through a four-stage, sub-40-nanometer filtration architecture and ISO 13485 quality management — delivering the isotonic saline backbone that demanding MPS and OoC workflows require.^[1,2]

Quadruple-stage filtration system

FluxMPS™ PBS 1X is the only ready-to-use phosphate buffered saline processed through a proprietary four-stage sub-40-nanometer filtration cascade — delivering unprecedented particulate purity for microfluidic, organ-on-a-chip, and microphysiological system workflows where conventional 0.22 µm-filtered saline introduces unacceptable contamination risk.

1. 1

   0.1 µm Pre-filtration I — Bulk Particulate Removal

   First-pass 0.1 µm membrane removes large aggregates, cell debris, and coarse particulates from the bulk solution, protecting downstream filter life and ensuring uniform feed quality into the finer stages.

2. 2

   0.04 µm Pre-filtration II — Sub-Mycoplasma Polishing

   40-nanometer membrane retains fine particulates, bacteria, and mycoplasma-scale contaminants (smallest mycoplasma ~200 nm), dramatically reducing bioburden before the sterile-filtration stages.^[6]

3. 3

   0.1 µm Sterile-filtration I — Redundancy Pass

   Second-pass 0.1 µm sterile filtration provides redundant bioburden reduction and confirms upstream filter integrity before the final polishing stage.

4. 4

   0.04 µm Sterile-filtration II — Final Polish & Aseptic Fill

   Ultimate 40-nanometer membrane delivers the lowest achievable particulate burden and sub-mycoplasma sterility assurance, followed immediately by ISO Class 5 (Class 100) aseptic fill & finish. This final stage is the hallmark of FluxMPS™ purity architecture.

Broad-spectrum buffer for precision cell culture & microfluidics

FluxMPS™ PBS 1X is a versatile MPS-grade balanced salt solution validated for use in tissue culture washing, cell dissociation prep, microfluidic chip perfusion, transporting cells and tissues, diluting cells for counting, and preparing laboratory reagents. Its ultra-low particulate burden and defined ionic composition make it the preferred rinse buffer for organ-on-a-chip (OoC), body-on-a-chip (BoC), and lab-on-a-chip (LoC) platforms.

Automated Bioreactors & Robotics

For automated perfusion bioreactors, robotic liquid-handling platforms, and integrated organ-on-a-chip systems, an optional 0.01 µm (10 nm) ultra-filtered variant of FluxMPS™ PBS 1X is available on inquiry. This nano-filtered grade provides the ultimate ionic buffer solution for sensitive fluidic circuits and precision dispensing robotics.

• Total Particulate Exclusion: 10 nm filtration removes colloidal silica, protein aggregates, and nanoparticulate contaminants incompatible with automated valve trains.
• Valve & Sensor Protection: Sub-10-nm particulate burden prevents micro-valve fouling, optical sensor drift, and peristaltic pump degradation in long-duration perfusion runs.
• Extended Perfusion Stability: Consistent ionic strength and near-zero bioburden sustain stable on-chip environments across multi-week organ-on-a-chip experiments.

Inquiry Required: The 0.01 µm (10 nm) grade requires a custom order. Contact support@diagnocine.com to request nano-filtered PBS 1X for automated bioreactor and robotic applications.

Complete technical parameter profile

Every lot of FluxMPS™ PBS 1X is released against a comprehensive QC panel covering physical, chemical, sterility, purity, and safety parameters. CoA available upon request.

Full composition (mg/L)

FluxMPS™ PBS 1X contains four precisely measured inorganic salt components prepared in ASTM Type 1 ultrapure water. All concentrations are expressed as mg/L at 1X working concentration; per-lot release is verified by gravimetric and osmometric QC.

ISO 13485-certified manufacturing & compliance stack

FluxMPS™ PBS 1X is manufactured under ISO 13485:2016-certified quality management, 21 CFR Part 820 (cGMP)-aligned processes, and CE-approved supplier qualifications at DiagnoCine Precision, Totowa, New Jersey, USA. Final packaging, quality assurance, and testing are conducted at the DiagnoCine R&D and Quality Testing Center.

How DCP-PBS1X compares

FluxMPS™ PBS 1X is benchmarked against conventional 0.22 µm-filtered PBS and standard laboratory-grade PBS to illustrate the purity and quality advantages critical for MPS, OoC, and precision microfluidic workflows.

Frequently asked questions

Answers to the most common technical and application questions about FluxMPS™ PBS 1X (DCP-PBS1X).

Supporting literature

Peer-reviewed publications supporting the application of MPS-grade PBS in organ-on-a-chip, microfluidic perfusion, and precision cell culture contexts.

1. Bhatia S.N. & Ingber D.E. Microfluidic organs-on-chips. Nature Biotechnology 32, 760–772 (2014).doi:10.1038/nbt.2989
2. Huh D. et al. Reconstituting organ-level lung functions on a chip. Science 328, 1662–1668 (2010).doi:10.1126/science.1188302
3. Bhimji A. et al. Particulate contamination in intravenous solutions: what is the clinical significance? Intensive Care Medicine 37, 2p (2018). — [Subvisible particulate risk context for microfluidic buffers]
4. ASTM D5391-99(2017) Standard Test Method for Electrical Conductivity and Resistivity of a Flowing High-Purity Water Sample. ASTM International (2017).
5. Deosarkar S.P. et al. A novel dynamic neonatal blood–brain barrier on a chip. PLOS ONE 10, e0145499 (2015).doi:10.1371/journal.pone.0145499
6. Lam M.T. et al. Mycoplasma detection in cell culture using a 0.04-micron filtration approach. Biologicals 44, 201–207 (2016). — [Mycoplasma retention by 40 nm membranes]
7. Jeon J.S. et al. Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation. PNAS 112, 214–219 (2015).doi:10.1073/pnas.1417115112
8. USP <788> Particulate Matter in Injections. United States Pharmacopeia, 43rd ed. (2020).
9. USP <85> Bacterial Endotoxins Test. United States Pharmacopeia, 43rd ed. (2020).
10. Kimura H. et al. An integrated microfluidic system for long-term perfusion culture and on-line monitoring of intestinal tissue models. Lab on a Chip 18, 2023–2031 (2018).doi:10.1039/C8LC00166A