OptiVitro 293 Cell Culture Medium HE03, Serum-free, dry powder

OptiVitro 293 Cell Culture Medium HE03, Serum-free, dry powder  

Cat. No.: EC-HE000-N023
Size: 50L


Description

OptiVitro® 293 Cell Serum-free Medium HE01 (powder) is an animal component-free, chemically defined (CD) medium that has been developed for the expansion of HEK293-derived suspension cell lines such as suspension 293T, 293F cells. OptiVitro® 293 Cell Serum-free Medium HE01 (powder) supports the growth and transfection of multiple suspension 293 cell lines, and can achieve high antibody/protein yields or high lentivirus/AAV/ADV titers when applied for protein expression or virus production. This product is free of antibiotics, and is manufactured in compliance with GMP regulations. OptiVitro® 293 Cell Serum-free Medium HE01 (powder) should be supplemented with L-Glutamine, the recommended working concentration is 6 mM.


Prepare media

1. 1.Take the preparation of 1L liquid medium as an example.
2. 2.Add about 800 mL water to a clean vessel.
3. 3.Add 22.73 g OptiVitro® 293 Cell Serum-free Medium HE01 (powder) slowly to the vessel, mix for 30-40 minutes.
4. 4.Slowly add 4.5 mL of 5 mol/L NaOH solution, adjust the pH to between 8.5 and 8.8, mix for 10 minutes.
5. 5.Add 2.317 g sodium bicarbonate powder slowly to the vessel, mix for 10 minutes.
6. 6.Slowly add 3.5 mL of 6 mol/L HCl solution, adjust the pH to between 7.0 and 7.2, mix for 10 minutes.
7. 7.Adjust the final volume to 1L, continue to stir for an additional 5 minutes.
8. 8.Measure and record the final osmolality. Expected values: 280 to 320 mOsm/kg.
9. 9.Sterilize by 0.22μm PES membrane filtration. Stored for 12 mouths at 2°C to 8°C away from light.

• Before use, add sterile L-glutamine solution to the liquid medium, the working concentration of L-glutamine is 6 mM. After L-glutamine is added, the medium is good to use within one week.