PBS, 1X (Phosphate Buffered Saline), Sterile | 40nm Ultrapure
Product Code: DCP-PBS1X-EX
Filtration: 0.04 micron (40nm) Double-Filtered
Grade: Tissue Culture & Molecular Biology Grade (RUO)
The Cleanest Buffer for Advanced Research
DiagnoCine Precision offers the cleanest buffer solution on the market, engineered to exceed the rigorous standards of modern biotechnology. While the industry standard is 0.22μm, our PBS is 0.04 micron (40nm) double-filtered, effectively removing sub-micron particulates, mycoplasma, and large viruses that interfere with sensitive assays.
Optimized for "On-a-Chip" Technologies
This high-precision formulation is specifically designed for fluidic stability and cellular health in micro-scale environments, including:
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Organ-on-a-Chip (OoC) & Tissue-on-a-Chip (ToC): Ensures a pristine environment for organoid development and long-term tissue maintenance.
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Body-on-a-Chip (BoC): Provides consistent physiological buffering across multi-organ integrated systems.
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Lab-on-a-Chip (LoC): Minimizes background noise and prevents micro-channel clogging in analytical devices.
Core Benefits for Researchers
- Maximum Purity: Sterilized by filtering TWICE (2X) through 0.04-micron (40nm) membrane filters in a controlled cleanroom environment—significantly cleaner than the industry-standard 0.22μm filtration.
- Optimal for Dissociation: Formulated without Calcium CaCl2 (-) and Magnesium MgCl2 (-) to prevent cell clumping and ensure high-efficiency rinsing before protease treatments.
- Superior Water Quality: Prepared using ASTM 0.05 μS/cm Type 1 Ultrapure Deionized H2O.
- Contaminant-Free: Guaranteed free of Protease, DNAse, RNAse, and Endonucleases.
Technical Specifications
| Property |
Value / Specification |
| Concentration |
1X (Ready-to-use) |
| pH Level |
7.4 (Customizable upon request) |
| NaCl |
137 mM |
| KCl |
2.7 mM |
| Phosphate Buffer |
8 mM (Na2HPO4), 2 mM (KH2PO4) |
| Form |
Sterilized Liquid, Clear, Colorless |
| Storage |
Room Temp (Short-term) / 4°C (Long-term) |
Applications
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Cell Culture: Washing, transporting, and diluting cells for counting.
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Reagent Prep: High-precision dilution of molecular biology reagents.
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Tissue Processing: Maintaining physiological pH and osmotic pressure for sensitive tissue samples.
Quality Assurance & Logistics
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Integrated Manufacturing: Fully produced, quality tested, and packaged at our DiagnoCine Precision facility in Totowa, New Jersey, USA.
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Certified Excellence: Operations are conducted under ISO 13485:2016 certified quality management systems.
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Shipping: Usually ships within 24 hours
Technical Comparison: 40nm vs. 0.22μm Filtration
Most commercial PBS utilizes 0.22μm (220nm) filtration. The Diagnocine 40nm filtered PBS offers significant advantages for sensitive applications:
| Feature |
Diagnocine (40nm) |
Standard PBS (0.22μm) |
| Pore Size |
0.04 micron (40nm) |
0.22 micron (220nm) |
| Filtration Level |
Double-Filtered (2X) |
Single-Filtered (1X) |
| Sterility Assurance |
Removes smaller mycoplasma
& large viruses |
Standard bacterial removal |
| Particulate Matter |
Minimal; ideal for Nano-science |
May contain sub-micron debris |
| Application |
High-res imaging & Microfluidics |
General lab washing |
Frequently Asked Questions (FAQ)
Why is 0.04 micron (40nm) filtration better than the industry standard?
Standard 0.22μm (220nm) filters are designed to remove bacteria but may allow smaller mycoplasma, viruses, and sub-micron debris to pass through. By using a 0.04 micron filter, we provide the cleanest buffer possible, which is essential for preventing interference in nano-science and high-resolution imaging.
Is this PBS safe for delicate Organ-on-a-Chip (OoC) systems?
Yes. In OoC, ToC, and LoC applications, even tiny particles can cause blockages or signal interference. Our double-filtration process ensures that the fluidic channels remain clear and the cellular microenvironment remains free of nano-particulate contaminants.
Can I use this buffer for Body-on-a-Chip (BoC) multi-organ integrations?
Absolutely. Because our PBS is prepared with Type 1 Ultrapure Water and is strictly controlled for pH and osmolarity, it provides the reliability needed for complex, multi-organ BoC systems where biological stability is paramount.
Is the solution ready to use right out of the bottle?
Yes. It is a ready-to-use 1X solution. You do not need to dilute it, which maintains the 40nm purity level and ensures that no contaminants are introduced during lab preparation.
