Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
World’s cleanest buffer for all cell and molecular biology experiments
Description:
MOPS Running Buffer [10X] is a concentrated solution commonly used in molecular biology and biochemistry, particularly for RNA gel electrophoresis. It contains 200 mM MOPS (3-(N-morpholino)propanesulfonic acid), 20 mM sodium acetate, and 10 mM EDTA, with a pH of 7.0. This buffer provides a stable pH environment, optimizing the separation of RNA molecules based on size and maintaining their integrity during electrophoresis. The 10X concentration allows for easy dilution to the working concentration of 1X, offering convenience and flexibility in preparation. MOPS Running Buffer is typically sterilized by filtration through a 0.04-micron filter and is guaranteed to be DNase and RNase-free, ensuring the protection of RNA samples from degradation. It is ideal for use with formaldehyde-agarose gels, Northern blot analysis, and as a running buffer for denaturing agarose gel electrophoresis of RNA. The use of MOPS buffer in formaldehyde-agarose gels significantly improves the resolution of large RNA species, particularly those above 6 kb.
Applications:
RNA gel electrophoresis: MOPS buffer is widely used for separating RNA samples on agarose and formaldehyde-agarose (denaturing) gels.
Northern blot analysis: It's utilized in the hybridization of total RNA or mRNA to membranes.
SDS-PAGE: MOPS buffer is commonly used for protein electrophoresis, particularly with Bis-Tris gels.
Western blotting: It facilitates the transfer of proteins from gels to membranes for specific protein detection and analysis.
Culture media: MOPS is used to regulate the acid-base balance in cell culture media, providing a suitable environment for cell growth.
UV/VIS spectrophotometry: MOPS is used to measure absorption in spectrophotometric analyses.
Advantages:
Zwitterionic nature: As a zwitterionic buffer, it maintains consistent ionic strength across its useful pH range.
Metal ion compatibility: Lacks the ability to form complexes with most metal ions, making it suitable for solutions containing metal ions.
Protein separation: Allows proteins to run slower compared to MES buffer, improving resolution for larger proteins.
Composition:
Ingredients
Concentration
MOPS
200 mM
Sodium Acetate
20 mM
EDTA
10 mM
Quality Control:
Appearance: Clear, Colorless Liquid.
pH: 7.0
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Storage: Room temperature
Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
