Hygromycin B (100 mg/mL)
Cat No.: DCP-HMB2G
Size: 20 mL (20 x 1mL)
Sterile; Ultrapure, Filtered 0.22-micron membrane Once.
Description:
Hygromycin B Solution (100 mg/mL in HEPES) is a ready-to-use, sterile aminoglycoside antibiotic formulation optimized for robust selection and maintenance of hygromycin-resistant cells in mammalian, plant, fungal, bacterial, and yeast systems. The antibiotic is produced by Streptomyces hygroscopicus and supplied as an active 100 mg/mL solution in sterile HEPES, ensuring excellent physiological compatibility and minimal impact on culture conditions.
Hygromycin B is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with ribosomal translocation and inducing misreading of the mRNA template. It stabilizes tRNA binding at the ribosomal A-site, prevents proper mRNA/tRNA translocation, and ultimately blocks polypeptide chain elongation, leading to rapid cell death in non-resistant cells. Resistance is conferred by hygromycin B phosphotransferase, encoded by hyg/hph genes, which inactivate the antibiotic via phosphorylation and allow selective survival of successfully transfected or transduced cells.
* Do not autoclave: Hygromycin B solutions must not be autoclaved as this will destroy antibiotic activity.
Applications
- Selection of stable transfectants or transduced cells expressing hygromycin-resistance markers.
- Maintenance of established hygromycin-resistant mammalian, insect, plant, fungal, yeast, or bacterial cell lines.
- Dual-selection strategies in combination with G418, blasticidin S, or other antibiotics for generating multi-resistant cell lines.
- Gene expression, vector integration, and genome-editing studies (including CRISPR/Cas9) requiring stringent, long-term selection.
- Antiviral research, exploiting hygromycin B’s ability to selectively enter virus-infected cells with increased membrane permeability and inhibit translation.
Advantages:
- 100 mg/mL Hygromycin B in HEPES, sterile-filtered and ready to use.
- Broad-spectrum activity against bacteria, fungi, and higher eukaryotic cells lacking hygromycin resistance.
- Ideal for selection and long-term maintenance of cells carrying hygromycin resistance genes (hyg or hph).
- Compatible with CRISPR/Cas9 and other genome-editing workflows requiring stable integration and selection.
- Manufactured as a cell culture–tested solution with high purity and validated potency.
Quality Control:
- Appearance: Clear, Colorless Liquid.
- pH: 7.4
- Sterility: Filtered 0.22-micron membrane Once.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: 4 ℃ in the dark.
- Shelf life: 2 years
- Do not freeze.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
