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Human carbonhydrate antigen 19-9 (CA19-9) ELISA kit
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Human carbonhydrate antigen 19-9 (CA19-9) ELISA kit
Sandwich quantitative immunoassay for carbonhydrate antigen 19-9 (CA19-9) in human serum, plasma available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | carbonhydrate antigen 19-9 (CA19-9) |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma |
| Detection range | 10 U/mL-150 U/mL |
| Sensitivity | 3.8 U/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, carbonhydrate antigen 19-9 (CA19-9) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 10 U/mL-150 U/mL with a minimum detectable dose of 3.8 U/mL.
This Human CA19-9 ELISA Kit was designed for the quantitative measurement of Human CA19-9 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 10 U/mL-150 U/mL and the sensitivity is 3.8 U/mL.
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<20% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
U/ml | OD1 | OD2 | Average |
| |||
150 | 2.045 | 2.011 | 2.028 |
| |||
100 | 1.292 | 1.300 | 1.296 |
| |||
40 | 0.728 | 0.735 | 0.732 |
| |||
10 | 0.304 | 0.306 | 0.305 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of human CA19-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 96 | ||||
Range % | 90-110 | |||||
1:2 | Average % | 89 | ||||
Range % | 85-99 | |||||
1:4 | Average % | 93 | ||||
Range % | 87-101 | |||||
1:8 | Average % | 95 | ||||
Range % | 89-98 | |||||
The recovery of human CA19-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 97 | 93-100 | ||||
EDTA plasma (n=4) | 97 | 90-101 | ||||