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Human Estradiol,E2 ELISA Kit
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* all types of species
* HRP and AP detection
* Low target signal enhancement
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4 core reagents below are included with every order, plus 1 of the 2 detection-specific reagents (matched automatically to your kit type) — 5 components, every time.
+ the 5th component, matched to your kit's detection method:
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All buffers and detection reagents work seamlessly across target proteins, bridging the gap in assay consistency.
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Human Estradiol,E2 ELISA Kit
Competitive quantitative immunoassay for Human Estradiol,E2 in human serum, plasma. available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma. |
| Detection range | 50 pg/ml-1200 pg/ml. |
| Sensitivity | 40 pg/ml. |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Competitive (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Signal Transduction |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Competitive binding
450 nm detection
Standard curve
Quantification
In this competitive ELISA, free Human Estradiol,E2 in the sample competes with plate-bound antigen for antibody binding. Signal intensity is inversely proportional to analyte concentration. Final quantification is derived from a standard curve fitted using Curve Expert software, covering 50 pg/ml-1200 pg/ml. with a minimum detectable dose of 40 pg/ml..
The human Estradiol (E2) ELISA kit is a powerful tool for quantitative measurement of estradiol levels in human serum and plasma samples, with a detection range of 50 pg/ml to 1200 pg/ml and a sensitivity of 40 pg/ml. The assay principle is competitive, meaning that two antibodies competitively bind to the estradiol in the sample. The detection is read at a wavelength of 450 nm. The assay time typically ranges from 1 to 5 hours, requiring a sample volume of 50-100 μl. The kit's performance has been validated in multiple studies found in more than 18 publications, ensuring its accuracy and reproducibility in measuring estradiol levels in human samples.
Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | ||||
0 | 1.952 | 1.834 | 1.893 | ||||
50 | 1.493 | 1.567 | 1.530 | ||||
120 | 1.045 | 1.028 | 1.037 | ||||
250 | 0.679 | 0.629 | 0.654 | ||||
500 | 0.419 | 0.421 | 0.420 | ||||
1200 | 0.234 | 0.243 | 0.239 | ||||
- A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit E2 antibody.
- Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- Anti-mouse E2 Antibody 1 x 6 ml -- Act as the detection antibody.
- HRP-conjugate 1 x 6 ml -- Bind to the E2 antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
- Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
- Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
- Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
- Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells)
- An Instruction manual
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
