SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
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Human Collagen Type I Precursor (Pro-Col Ⅰ)
Cat.No.: JNB-JL48539
Size: 96T
Species: Human
Assay Type: Sandwich
Sensitivity: 38pg/mL
Detection Range: 78-5000pg/mL
Alternative Names: Pro-Col Ⅰ
Research Areas: Cardiovascular Biology
Assay Time: 2h45m
Abbreviation: Pro-Col Ⅰ
Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Pro-Col Ⅰ. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Human Pro-Col Ⅰ in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Human Pro-Col Ⅰ in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human Pro-Col Ⅰ in samples. No significant cross-reactivity or interference between Human Pro-Col Ⅰ and analogues was observed.