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Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT
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Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT
Sandwich quantitative immunoassay for Collagen Type Ⅲ,Col Ⅲ in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | Collagen Type Ⅲ,Col Ⅲ |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 4.69 ng/mL-300 ng/mL |
| Sensitivity | 2.345 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Signal Transduction |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Collagen Type Ⅲ,Col Ⅲ in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 4.69 ng/mL-300 ng/mL with a minimum detectable dose of 2.345 ng/mL.
This ELISA kit provides quantitative measurement of Collagen Type III (Col III) in human serum, plasma, and tissue homogenates, supporting research into extracellular matrix remodeling and fibrotic signaling pathways. Because Col III turnover fragments and propeptides fluctuate across stages of tissue fibrosis, wound healing, and vascular remodeling, researchers need an assay calibrated to capture both baseline and disease-elevated concentrations across diverse sample matrices.
Detection Range: The 4.69–300 ng/mL range spans the physiological concentrations of circulating Col III-related fragments reported in healthy individuals through the markedly elevated levels seen in hepatic fibrosis, systemic sclerosis, and post-surgical wound repair, minimizing the need for extensive sample dilution in most serum and plasma applications.
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 | ||||||
ng/ml | OD1 | OD2 | Average | Corrected | ||
300 | 3.149 | 3.289 | 3.219 | 3.150 | ||
150 | 2.182 | 2.139 | 2.160 | 2.091 | ||
75 | 1.094 | 1.147 | 1.121 | 1.052 | ||
37.5 | 0.631 | 0.632 | 0.631 | 0.563 | ||
18.75 | 0.359 | 0.355 | 0.357 | 0.288 | ||
9.38 | 0.225 | 0.233 | 0.229 | 0.160 | ||
4.69 | 0.156 | 0.154 | 0.155 | 0.086 | ||
0 | 0.070 | 0.067 | 0.069 |
| ||
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 98 | ||||
Range % | 91-103 | |||||
1:2 | Average % | 93 | ||||
Range % | 88-100 | |||||
1:4 | Average % | 104 | ||||
Range % | 96-110 | |||||
1:8 | Average % | 88 | ||||
Range % | 81-98 | |||||
The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 89-100 | ||||
EDTA plasma (n=4) | 99 | 93-103 | ||||
