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Human Chorionic Gonadotrophin,HCG ELISA Kit
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Human Chorionic Gonadotrophin,HCG ELISA Kit
Sandwich quantitative immunoassay for Chorionic Gonadotrophin,HCG in human serum, plasma, urine, cell culture supernates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | Chorionic Gonadotrophin,HCG |
| Uniprot No. | P0DN86 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, urine, cell culture supernates |
| Detection range | 8 mIU/mL-240 mIU/mL |
| Sensitivity | 2 mIU/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Chorionic Gonadotrophin,HCG in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 8 mIU/mL-240 mIU/mL with a minimum detectable dose of 2 mIU/mL.
This Human hCG ELISA Kit was designed for the quantitative measurement of Human hCG protein in serum, plasma, urine, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 8 mIU/mL-240 mIU/mL and the sensitivity is 2 mIU/mL.
- Increases in early beta-chorionic gonadotropin levels are slower in vanishing twins than in singleton and twin pregnancies. PMID: 23541408
- In pregnant women with inherited thrombophilia, PAPP-A levels were significantly higher and fbHCG levels were lower during the first trimester. PMID: 23795618
- LC-MS/MS provided in-depth information on hCG variant composition of hCGn (99/642) and hCGbetan (99/692) and hCG variant specificity profiles and facilitated precise classification of the epitopes PMID: 24014048
- First trimester hCGbeta was not associated with placenta accreta. PMID: 24226752
- Maternal serum hCGB was not associated with fetal open spina bifida. PMID: 24226839
- Unlike the bovine c-terminal peptide of HCG-beta that lacks O-glycans and determinants for circulatory survival, human HCG-beta has extended plasma half-life. PMID: 24239648
- no association between prior pre-eclampsia and first trimester pregnancy level PMID: 24304164
- Time-to-pregnancy >/=24 months in spontaneously conceived pregnancies is associated with decreased levels of PAPP-A and free beta-hCG. PMID: 24338604
- Hyperglycosylated CGB is superior to traditional hCG for predicting pregnancy outcome after in vitro fertilization. PMID: 24355054
- Initial human chorionic gonadotropin values did not predict treatment success in cases with complete hydatidiform mole. PMID: 24379071
- Majority of the patients in 15 cases of hCG positive CSs without typical syncytiotrophoblastic cells showed elevated level of human chorionic gonadotropin (hCG)in blood preoperatively. PMID: 24480432
- results of the experiments showed that hCGbeta action leads to the increase in ERK and AKT kinases phosphorylation in cancer cells and indicate that these biological effects can be achieved independently of human chorionic gonadotropin presence PMID: 24554412
- may directly stimulate angiogenesis PMID: 24560494
- Case Report: Krukenberg tumor presenting as back pain and a positive urine pregnancy test due to elevated urinary CGB. PMID: 24708577
- CGB genes 1 and 2, previously considered as pseudogenes, are notably expressed by epithelial cancer cell lines. PMID: 24778026
- Expression of beta-hCG may be seen in high-grade osteosarcoma, but frequent beta-hCG immunohistochemical expression by tumor cells, associated with clinically elevated serum beta-hCG, is rare. PMID: 24856811
- CGB - core fragment was isolated from the urine of women, pregnant with normal, molar and hyperemesis gravidarum pregnancies. Larger glycoforms were more abundant in the abnormal pregnancies. PMID: 24905406
- The concentration of cell-free beta-HCG mRNA in maternal plasma may be applicable to the prenatal diagnosis of placenta accreta. PMID: 25063251
- During pregnancy, regulation of hCG beta subunit genes expression correlates with both methylation of their promoters and TFAP2A expression level. PMID: 25063728
- Hyperglycosylated human chorionic gonadotropin is associated with gestational trophoblastic diseases PMID: 25275660
- CGB was higher in pregnant women with chronic renal insufficiency compared to controls and women with renal disease and normal renal function. PMID: 25395363
- Data suggest that initial serum beta-HCG levels in women after transfer of vitrified--warmed cleavage-stage embryos are lower than those after transfer of slow cooled--thawed cleavage-stage embryos. PMID: 25592975
- Median free betahCG MoM in the control group was 1.04, and 1.08 (P = 0.859) in the placenta praevia group compared with 0.81 in the abnormally invasive placentation group (P = 0.06). PMID: 25639820
- Depending on its source of production, glycoforms of hCG display different biological activities and functions that are essential for pregnancy outcome.[review] PMID: 25707740
- placenta-mediated adverse pregnancy outcome (PMAPO) before term but not PMAPO at term associated with elevated serum level in second trimester PMID: 25767942
- For Down syndrome affected pregnancies, the median MoM of first trimester PAPP-A, PlGF, and AFP were significantly reduced, and free-beta-hCG were significantly elevated. PMID: 25846403
- hCGB serum concentrations differed significantly between Asian and European women during the first trimester of pregnancy. PMID: 25858516
- Data suggest that low serum levels of hCGB/ADAM12 are biomarkers in diagnosis of continued incomplete spontaneous abortion; here, hCGB/ADAM12 indicate success/failure of single dose of misoprostol/oxytocic in treatment of early pregnancy failure. PMID: 26051455
- reveals a positive feedback loop between glial cells missing 1 and Human chorionic gonadotropin regulating placental hCGbeta expression and cell differentiation PMID: 26503785
- All pregnancies with serious complications had serum beta-human chorionic gonadotropin levels of 1.0-5.0 mIU/ml. PMID: 26702770
- Data indicate the diagnostic criteria of chorionic gonadotropin beta subunit (beta-hCG) and alpha-Fetoprotein (alphaFP) in CSF and serum, which may advance the early and formal diagnosis of intracranial germ cell tumors (ICGCTs). PMID: 26968839
- Serum hCG-beta levels of POD 12 and 14 and the fold increase of hCG-beta levels from POD 12 to 14 significantly predict pregnancy outcomes after fresh and frozen cycles. PMID: 27262839
- the results of this study provide novel evidence supporting a role of hCG in regulating the differentiation of peripheral FOXP3(+) Tregs, which affect the pregnancy outcomes of patients with a history of recurrent implantation failure PMID: 28044377
- Chorionic Gonadotropin, beta Subunit promoted the migration and invasion of CRC in vitro and in vivo but had no effect on the proliferation of tumor cells. PMID: 29357321
- risk for giving birth to a child with cerebral palsy was higher in women who did not have high hCG concentrations in the 1st trimester and low hCG concentrations in the 2nd trimester PMID: 30007247
- The presence of hCGbeta positive tumor cells appears to potentially reflect the aggressive behavior of mucoepidermoid carcinoma and adenoid cystic carcinoma PMID: 30197330
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of human HCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| The recovery of human HCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
