Heparin-GNP
Product#: FNK-GC-HEP-500
$577.97
Availability:
Ships in 1-2 Weeks
Heparin-GNP
Cat. No. FNK-GC-HEP-500
Store at 4°C
Size 500µl
Description
SGNP（Sugar chain - immobilized Gold Nano-Particle） is a gold nano particles immobilized with structurally defined sugar chains, showing red-purple color (λmax = ca. 530 nm). SGNP is a very convenient tool for evaluating sugar chain – protein interaction, since the interaction can be detected visually, and can be quantified from the change of OD at 530 nm. There are several applications as follows:
- Aggregation of protein
- Inhibition assay
- Dot-blotting
- Isolation and purification of lectin from a crude extract
Regularly sugar chain binding proteins, such as lectins, possess multiple binding sites for sugar chains. By the addition of SGNP to the protein solution, the protein may form aggregates with sugar chains immobilized SGNP in a short time of period.
The change can be seen visually, or can be quantified by measuring OD at around 530 nm. Using SGNP, the binding properties (selectivity, dissociation constant (KD), specificity, etc.) are easily evaluated.
Example
Example
Application#2: Inhibition assay
The sugar chain binding potency of the trace amount of your samples can be detected.
* Independent on the valency of sugar-binding proteins.
* Independent on the valency of sugar-binding proteins.
Quick isolation and identification of protein(s) having multi-binding sites (lectin) from a plant-derived crude extract of cell-lysate are available using SGNP. Once you get an aggregate by mixing appropriate SGNP with your extract or lysate, the aggregate can be directly applied for SDS-PAGE. Then, the protein band(s) in SDS-gel is analyzed according to the proteomics procedure.
SGNP (Sugar chain immobilized Gold Nano Particle)
SGNPs
SGNP set
