HRPQuench-TBS

Cat No.: DCP-HRPTBS1X

Size: 100 mL

Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.

HRPQuench-TBS represents a significant advancement in horseradish peroxidase (HRP) signal management for Tris-buffered saline-based immunoassay systems. This advanced formulation, engineered with a unique combination of enzyme inhibitors and stabilizing agents, effectively neutralizes HRP activity between multiple staining cycles, providing precise control in complex, multi-step immunolabeling protocols. HRPQuench-TBS ensures complete HRP inactivation without affecting tissue morphology or subsequent antibody-antigen interactions. By eliminating unwanted HRP activity and minimizing background staining, this solution enhances the accuracy and reliability of multiplex immunohistochemistry and immunofluorescence techniques. Particularly beneficial for protocols involving multiple HRP-conjugated antibodies, HRPQuench-TBS enables distinct, non-overlapping labeling of various targets within a single specimen. Its optimized composition not only improves staining specificity but also streamlines workflows, reducing overall assay time and reagent consumption, making it an essential tool for advanced immunological research.

Applications:

• Multiplex immunohistochemistry (mIHC) with sequential HRP-based staining cycles

• TSA-based multiplex immunofluorescence workflows

• Endogenous peroxidase suppression prior to chromogenic or fluorescent detection

• Multi-round IHC protocols using same-species primary antibodies

• Automated IHC/IF staining platforms

Advantages:

• Complete HRP Inactivation — prevents signal carryover between staining rounds

• Tissue & Antigen Safe — does not alter tissue morphology or disrupt subsequent antibody binding

• Cleaner Results — reduces non-specific background in both chromogenic and fluorescent assays

• TBS-Optimized — compatible with downstream immunoreagents at pH 7.4–7.6

• Easy to Use — room temperature incubation, no special equipment needed

• Versatile — validated for FFPE and frozen tissue sections

Composition:
 

Quality Control:

• Appearance: Clear, Colorless Liquid.

• pH: 7.4

• Sterility: Non-Sterile

Storage and Shelf Life:

• Storage: 4℃ 

• Shelf life: 1 year

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.