Formaldehyde Gel Loading Buffer [10X]
Cat No.: DCP-FGL10X
Size: 2 x 1 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
The 10X Formaldehyde Gel Loading Buffer is a specialized solution designed for RNA sample preparation in denaturing gel electrophoresis. It facilitates the loading process by increasing the density of the sample and providing visual tracking. EDTA acts as a chelating agent and inhibits metal-dependent nucleases, protecting RNA from degradation. Bromophenol blue and xylene cyanol serves as a tracking dye and allows visual monitoring of the electrophoresis progress with bromophenol blue typically running faster and xylene cyanol FF running more slowly. Glycerol increases the density of the sample and ensures that the RNA sample sinks to the bottom of the well when loaded. This buffer is crucial for maintaining RNA in a denatured state during gel electrophoresis, allowing for accurate size separation and analysis of RNA.
* Please note that this buffer contains bromophenol blue.
Advantages:
- Sample density: The high glycerol content (50%) increases the sample density, ensuring it sinks to the bottom of the well when loaded onto the gel, preventing sample loss and improving loading accuracy.
- Nucleic acid protection: EDTA (10mM) acts as a chelating agent, inhibiting metal-dependent nucleases and protecting RNA from degradation during electrophoresis.
- Visual tracking: The bromophenol blue (0.025%) and xylene cyanol FF (0.025%) serve as tracking dyes, allowing researchers to monitor the progress of electrophoresis visually.
Application:
- RNA gel electrophoresis: It is primarily used for preparing RNA samples for separation on formaldehyde-containing agarose gels.
- Northern blot analysis: The buffer is suitable for sample preparation in Northern blotting, which involves the hybridization of RNA to a membrane.
- Pre-rRNA and rRNA analysis: It aids in the separation and visualization of high molecular weight RNA species, such as ribosomal RNAs and their precursors.
- RNA integrity assessment: The buffer helps in preparing samples for evaluating RNA quality and integrity through gel electrophoresis.
Composition:
| Ingredients |
Concentration |
| Glycerol |
50% |
| Bromophenol blue |
0.025% |
| Xylene cyanol FF |
0.025% |
| EDTA |
10mM |
Quality Control:
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Appearance: blue-purple or violet color.
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pH: 8.0
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of other tracking dyes, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
