FluxMPS™ Click’s Medium (EHAA) Eagle Hanks’ Amino Acids w/o L-Glutamine, Sodium Bicarbonate, Mercaptoethanol, 1X Liquid Cell Culture

FluxMPS™ Click’s Medium (EHAA) Eagle Hanks’ Amino Acids w/o L-Glutamine, Sodium Bicarbonate, Mercaptoethanol

FluxMPS™ Click’s Medium (EHAA) is an MPS-grade, ultra-filtered cell culture medium engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic research. Purified through a proprietary Quadruple-Stage Filtration System (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× fewer subvisible particulates than conventional 0.22 µm-filtered media—making it the premier choice for microchannel-safe immune-cell culture and chip-based immunology assays.

• Nano-filtration at 0.04 µm final stage: sub-mycoplasma polishing for ultra-clean microchannels
• 4-stage quadruple filtration (0.1 µm ×2 + 0.04 µm ×2) — industry-leading purity architecture
• Endotoxin NMT 0.05 EU/mL, verified by Limulus Amebocyte Lysate (LAL/BET) assay per USP <85>
• Elevated essential and non-essential amino acid profile + nucleic acid precursors (Adenosine, Cytosine, Guanosine, Uridine) for immune-cell viability
• Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) for zero background interference
• ISO Class 5 (Class 100) aseptic fill & finish under ISO 13485:2016 QMS
• [+] Low Glucose (1.0 g/L), [+] Sodium Pyruvate; [−] L-Glutamine, [−] Sodium Bicarbonate, [−] Mercaptoethanol
• Custom pH, glucose, salts, HEPES & formulation modifications available on request

Engineered where standard media fails

Conventional 0.22 µm-filtered cell culture media harbour mycoplasma (≤0.2 µm), subvisible particulates, and residual endotoxin that accumulate inside microfluidic channels as narrow as 10 µm — corrupting cytokine measurements, blocking microvalves, and generating false-positive biosensor signals. For immune-cell studies in MPS and OoC platforms, where mouse spleen cells, primary lymphocytes, or iPSC-derived immune models must respond authentically to defined stimuli, medium purity is non-negotiable. FluxMPS™ resolves every failure mode of conventional media in a single ready-to-use formulation.^[1,2]

Quadruple-stage filtration system

FluxMPS™ Click’s Medium (EHAA) is the only ready-to-use Click’s formulation produced at 0.04 µm final filtration purity — a standard previously achievable only by on-site syringe filtration by the researcher. Each lot undergoes four sequential membrane filtration stages under ISO Class 5 (Class 100) aseptic conditions, delivering a particle burden approximately 5× lower than conventional 0.22 µm-filtered media by USP <788> particulate count.^[5]

1. 1

   0.1 µm Pre-filtration I

   Removes large particulates, aggregates, and cellular debris. Extends downstream membrane life and protects the precision 0.04 µm membranes from rapid fouling.

2. 2

   0.04 µm Pre-filtration II

   Fine-particulate removal and mycoplasma barrier. Retains all mycoplasma species (≤0.2 µm), bacteria, and subvisible particles before the sterile fill stage.

3. 3

   0.1 µm Sterile-filtration I

   Second-pass 0.1 µm redundancy filtration under ISO Class 5 conditions. Provides bioburden reduction confirmation prior to the final polishing stage.

4. 4

   0.04 µm Sterile-filtration II — Final Polish

   Ultimate purity polish and aseptic fill under ISO Class 5 (Class 100) laminar-flow environment. Achieves sub-mycoplasma, microchannel-safe purity with USP <788>-compliant particulate profile.

Where FluxMPS™ Click’s Medium (EHAA) performs

Originally developed by Robert Click to study the in vitro immune response of mouse spleen cells using heterologous red blood cells (RBCs), Click’s Medium (EHAA) has evolved into a versatile platform for immune-cell biology, metabolic research, and next-generation microphysiological systems. The FluxMPS™ ultra-filtered formulation extends its utility to microfluidic organ-on-a-chip platforms where conventional media purity is insufficient.^[1,6]

Automated Bioreactors & Robotics

For automated bioreactor platforms and robotic liquid-handling systems where media contacts precision valves, sensors, and tubing over extended perfusion runs, an optional 0.01 µm (10 nm) ultra-filtered variant of FluxMPS™ Click’s Medium (EHAA) is available on inquiry — providing the highest possible particle-exclusion for closed-loop automation.

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulates that pass 0.04 µm membranes, protecting optical sensors and flow sensors from signal drift.
• Valve & Sensor Protection: Prevents micro-valve fouling and micro-electrode contamination in long-duration perfusion experiments.
• Extended Perfusion Stability: Maintains media quality across multi-day automated perfusion cycles without nutrient degradation artefacts from particulate-induced oxidation.

Inquiry Required: The 0.01 µm grade is produced in micro-batches on request. Contact support@diagnocine.com to discuss your automation platform requirements and lead time.

Analytical release specifications

Every lot of FluxMPS™ Click’s Medium (EHAA) is analytically characterised and released against the specifications below before shipping. Certificate of Analysis (CoA) available on request at support@diagnocine.com.

Full composition (mg/L)

The complete formulation is reproduced below. Nucleic acid precursors (Adenosine, Cytosine, Guanosine, Uridine) distinguish Click’s Medium (EHAA) from standard Hanks’ salt media. All mg/L values are as-formulated at 1X concentration. L-Glutamine, Sodium Bicarbonate, and Mercaptoethanol are omitted by design; each is supplied as a separate supplement per researcher protocol. Each lot is released against composition targets confirmed at the DiagnoCine R&D and Quality Testing Center.

Manufacturing & compliance framework

FluxMPS™ Click’s Medium (EHAA) is produced under ISO 13485:2016 certified and CE-approved supplier facilities. All final packaging, quality assurance, and testing are performed at the DiagnoCine R&D and Quality Testing Center. All customisation and assembly is accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.

How DCP-CM-QB1X compares

FluxMPS™ Click’s Medium (EHAA) is the only commercially available Click’s EHAA formulation processed to 0.04 µm final filtration purity with a full Quadruple-Stage filtration architecture, verified by USP <788> particulate analysis and ISO 13485:2016 QMS release testing.

Frequently asked questions

Common questions about FluxMPS™ Click’s Medium (EHAA) (DCP-CM-QB1X), its filtration system, supplementation requirements, and quality documentation.

Supporting literature

Curated peer-reviewed publications relevant to Click’s Medium (EHAA), immune-cell biology, organ-on-a-chip research, and microphysiological system media requirements. In-text citations (^[n]) throughout this page correspond to the entries below.
This product has been utilized by researchers at MD Anderson Cancer Center in scientific and laboratory applications.

1. Click RE, Benck L, Alter BJ. Immune responses in vitro. I. Culture conditions for antibody synthesis in vitro by normal mouse spleen cells. Cell Immunol. 1972;3(2):264–276. doi:10.1016/0008-8749(72)90071-0
2. Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668. doi:10.1126/science.1188302
3. Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. 2016;164(6):1105–1109. doi:10.1016/j.cell.2016.06.005
4. Bhattacharya A, Bhattacharya S. Mycoplasma contamination of cell cultures: Incidence, implications, and management. Indian J Med Microbiol. 2021;39(4):493–500. doi:10.1016/j.ijmmb.2021.07.007
5. United States Pharmacopeia. USP <788> Particulate Matter in Injections. USP-NF. Rockville, MD: USP; 2023. doi:10.31003/USPNF_M74950
6. Sung JH, Esch MB, Prot JM, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13(7):1201–1212. doi:10.1039/c3lc51138h
7. Rothbauer M, Zirath H, Ertl P. Recent advances in microfluidic technologies for cell-to-cell interaction studies. Lab Chip. 2018;18(2):249–270. doi:10.1039/c7lc00815e
8. Luni C, Giulitti S, Serena E, Ferrari L, Zambon A, Gagliano O, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13(5):446–452. doi:10.1038/nmeth.3832
9. van Midwoud PM, Janse A, Merema MT, Groothuis GM, Verpoorte E. Comparison of biocompatibility and adsorption properties of different plastics for advanced microfluidic cell and tissue culture models. Anal Chem. 2012;84(9):3938–3944. doi:10.1021/ac300771z
10. Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol. 2007;8(10):839–845. doi:10.1038/nrm2236