Easy WESTERN Multi 50

Cat. No.: FNK-BCL-EZU01

Size: 25assays

Storage: -20℃ (MAD reagent) 4℃ (other reagents)


Description:

Easy-WESTERN (EZW) is the primary antibody-detecting kit designed for Western Blotting based on MAD (multi-antibody detection) or MAD-II technology.  With newly developed MAD-II, essentially all IgG subtypes of all mammal species can be detected, and you will get many advantages, such as high sensitivity, no requirement of second antibody, and quick detection.

Easy-WESTERN Multi uses MAD-II reagent as primary antibody detection achieving high sensitivity and quick detection.


Principle

There are two types of MAD probes, MAD or MAD-II. These probes are nano particles composed of  IgG-binding protein with each particle displaying 200 or more binding domain of the Fc region of IgG and labeled with about 50 HRP molecules. Because of high amount of IgG binding sites and labeled HRP, IgG are detected with high sensitivity. Though MAD showed weak affinity to a few IgG species, MAD-II shows good affinity of all IgG subtypes of all mammalian species  (all IgG subtypes of mouse, rat, rabbit, guinea pig, goat, horse, donkey, pig, bovine, sheep, dog, cat, monkey, human).


Product Characteristics

The basic components of Easy-Western are MAD regents and MAD dilution buffer. MAD is used as a substitute of 2nd antibody. MAD dilution buffer is used to dilute MAD to eliminate non-specific binding. When using Easy-Western, there are many kinds of ways depending on the purpose.  Some of examples are shown below.


1. High sensitivity detection without secondary antibody

With ordinary protocol just change primary antibody to MAD reagents.




2. One step detection with high sensitivity
Mix primary antibody with MAD reagents and react membrane with the mixture.




3. Save time by using One-Step detection
Mix primary antibody with MAD reagents and react membrane with the mixture using short time protocol.



4. Signal enhancement of weak signal using ordinary western
Membrane with weak signal  by ordinary secondary antibody method can be enhanced second reaction using MAD reagent.




5. Multiple antigen detection
By using multiple primary antibodies regardless animal species, multiple antigen detection is possible by a single procedure with MAD reagents.




6. Ultra high sensitivity by using MAD as third reaction
Using third reaction with MAD after 2nd antibody reaction.