DEPC(Diethylpyrocarbonate)-treated Water is a strong acylating agent that can be used in various applications in molecular biology experiments with use of RNA in samples. An acylating agent is one in which an acyl (RCO-) radical group is introduced into molecules of a compound. DEPC inactivates RNase enzymes in water by covalent modifications of the histidine residues. In the field of Molecular Biology, it is a powerful but not absolute inhibitor of RNase activity (i.e. ribonuclease inhibitor). DEPC not only inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose-time dependent manner but increases the Ca2+ permeability of Sarcoplasmic Reticulum (SR) vesicles. DEPC is also useful for specific inactivation of nucleases during isolation of undegraded polynucleotides as well as inhibiting the platelet-activating factor (PAF), acetylhydrolase. It has also been used as a probe for the topography of 5.8S rRNA yeast ribosomes. DEPC-Treated Water is suitable for all PCR reactions.
This product was prepared by adding 0.1 % DEPC to Ultrapure Type 1 water (endotoxin free, DNase/RNase free) and full cycle of autoclaving, sufficiently eliminating RNase in the water for all further molecular applications such as PCR. Although at times, even after sufficient autoclaving, a slight EtOH or fruity smell may linger which is caused by the EtOH by-product segueing with the trace carboxylic acid contaminates resulting in the formation of volatile esters. This, however, does not mean that traces of DEPC remain in the solution.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
Quality Control
Apperance: Clear, Colorless Liquid.
pH (at 1X concentration): 7.4
Sterility: Filtered in a sterile environment.
Storage and Shelf Life
Storage: 4 °C
Shelf life: 24 months
* All DiagnoCine Precision Sterile filtered-sterilized with 40 nanometer. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
