D35 Endotoxin-Free (A-class TLR9 Ligand )
D35 Endotoxin-Free (A-class TLR9 Ligand )
Cat. No. FNK-CN-65001
Size 10mg
Storage -20°C
Description
TLR9 Ligand (CpG-ODN) for pDCs and B-cells D35 and K3
CpG Oligodeoxy nucleotide (CpG-ODN), which contains CpG-motif, has a function to stimulate immune system through Toll-like receptor 9 (TLR9) and promote maturation and activation of antigen presenting cells (APCs). This mimics immuno-stimulation activity of bacterial DNA.
D35 is categorized in "A-class" TLR9 ligand. D35 strongly induces production of type I Interferon (IFN) by activating pDCs and PBMCs, but it shows lower activity to B-cells.
K3 is categorized in "B-class" TLR9 ligand. K3 induces activation of TLR9-dependent NF-kB signal by stimulating B-cells, but it shows lower activity for inducing IFN-alpha.
Common features of both D35 and K3
- Strong and Specific immune stimulation for human and mouse immune cells.
- High purity
- Endotoxin-free (<0.5 EU/mg)
- Can be used for in vivo study (*These items are for research use only)
- Bulk amount and GMP-grade product is available.
- A-class TLR9 ligand
- Induce production of type I Interfron (IFN) by activating pDCs and PBMCs,
- Lower activity to B-cells.
- B-class TLR9 ligand
- Induce production of Interleukin 6 (IL-6) from human PBMC
- Induce activation of TLR9-dependent NF-kB signal by stimulating B-cells
- Lower activity for inducing IFN-alpha.
Fig. 1 Cytokine induction from human PBMCs
Human PBMC (1 x 10^6 cells) were stimulated with each CpG (1 μM) for 24 hours.IFN-alpha and IL-6 in culture supernatant were measured by ELISA. Bar graph shows average +/- SEM.
Fig. 2 Cytokine induction from mouse FL-DCs
FL-DCs (2 x 10^6 cells) derived from mouse bone marrow were differentiated by FLT3L and stimulated by each CpG (1 μM) for 24 hours. IFN-alpha and IL-6 in culture supernatant were measured by ELISA. Bar graph shows average +/- SEM.
