Coomassie Bright Blue Ultrafast Staining, 500ML
Cat. No.: SB-G2059-500ML
Size: 500ML
Description
Coomassie brilliant blue ultrafast staining solution is a staining solution with Coomassie brilliant blue G250 as the dye. It can be used to stain protein gels such as SDS-PAGE or non-denaturing PAGE, and can also be used to detect residual proteins on PAGE glue after Western Blot. The staining solution does not contain toxic methanol and pungent odor of acetic acid in conventional staining solution; Pollution-free, non-toxic, non-irritating odor, highly environmental protection; The use of this staining solution does not require fixation, the staining time is shorter, and the sensitivity is high. For a 1.0 mm thickness gel, 50 ng of protein was detected after half an hour of staining; After 1 hour of staining, a minimum of 20 ng protein could be detected. The protein bands could be observed after washing with pure water for 30 min or without cleaning.
Storage and transportation
Transport at room temperature; Store at 4℃ away from light, valid for 12 months.
Assay Protocol
1. Take out the polyacrylamide gel after electrophoresis, put it into a clean vessel, clean it with pure water three times, 20 s each time；
2. Pour out pure water, add 20 mL of Coomassie brilliant blue ultrafast staining solution (based on submerged gel), and shock stain for 30 min (the gel staining time can be adjusted in real time according to the color band depth of protein)；
3. Pour out the staining solution and wash with pure water by shaking for 30 min. The longer the decolorization time, the lighter the background will be (adjust the decolorization time according to the experimental needs). If the gel with lower background is needed, the gel can be decolorized with pure water for more than 1 h or overnight.
Note:
1. There will be precipitate at the bottom of the bottle after a period of time. Please mix it upside down before use.
2. The staining solution is acidic and slightly corrosive. Please take protective measures.
3. Cleaning with pure water before dyeing can greatly reduce the interference of SDS.
4. Please take photos as soon as possible after dyeing. Long immersion in pure water will lead to the loss of protein and dye.
5. For your safety and health, please wear a lab coat and disposable gloves when operating.
