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Cellulase HT4, Thermostable

Product#: FNK-EGL-54-04

$354.22

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Cellulase HT4, Thermostable

Cat.No. FNK-EGL-54-04
Lot No. 0-H801B
Protein conc. 7.98 mg/ml
Volume 5 ml
Form 20 mM AcONa (pH 6.0), 50 mM NaCl, 1 mM DTT
Storage -20 oC *Avoid the freeze and thaw.
Activity 55.7 U/ml
Notes For research use only.

Description

Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

The enzyme solution was mixed with the pre-warmed substrate solution (0.5 % CMC, 0.1 M acetate-NaOH buffer (pH 6.0 @ 25°C)). After incubation at 70 °C for 5 min, the reducing power was measured by the modified Somogyi-Nelson methods.* One unit is defined as the amount of enzyme producing reducing power equivalent to 1 mg of glucose per min.
*Hiromi et al. (1963) Bull Chem Soc Jpn 36: 563-569