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Broad Range RNA Quantification Kit, 500 assays

Product#: BM-F1000-RBR-500

$365.00

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Broad Range RNA Quantification Kit, 500 assays

Cat. No.: BM-F1000-RBR-500

Description

The Accuris RNA Broad Range (BR) Quantification Kit provides a simple, accurate, and highly selective method for RNA quantification. Designed for specificity to RNA over single stranded (ssDNA) and double-stranded DNA (dsDNA) (see figure below), the kit enables reliable detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL.

This kit offers superior stability and sensitivity compared to other broad-range RNA quantification kits. Additionally, it is highly tolerant to common contaminants, including proteins, free nucleotides, salts, solvents, and detergents, ensuring consistent and accurate results. The assay is performed at room temperature. Simply dilute the BR RNA reagent in the RNA BR buffer, add samples and standards, and measure RNA concentration using a fluorometer or fluorescent plate reader. (For additional details on compatibility with fluorescent plate readers, please contact us.)

Kit Components & Storage Requirements

Material	Storage	Amount	Concentration	Stabiliy
Accuris RNA BR Reagent (Component A)	4 ℃ Protect from light	100 μL	200X in DMSO	Product Stable for at least 6 months when stored as directed
Accuris RNA BR Buffer (Component B)		20 mL	1X
Accuris RNA Standard #1 (Component C)		100 μL	0 ng/μL in TE Buffer
Accuris RNA Standard #2 (Component D)		100 μL	100 ng/μL in TE Buffer

Critical Assay Parameters:

Incubation Time: For optimal detection of ssDNA, incubate the samples and standards with the working solution for 2-3 minutes in the dark at room temperature.

Fluorometer Calibration: Users can either use the previous calibration or perform a new calibration. We recommend performing a new calibration for each assay to minimize deviations in the results.

Assay Temperature: The Accuris ssDNA Quantification Kit offers optimal performance at room temperature. Therefore, all reagents must be equilibrated to room temperature before use.

Effect of Contaminants: The Accuris ssDNA Quantification Kit is tolerant to common contaminants such as proteins, salts, solvents, and detergents.

Figure: Sensitivity of Accuris ssDNA Quantification kits to ssDNA

Standards and Sample Preparation:

Protocol: Using the Accuris ssDNA Quantification Kit with Accuris Smart Q and Qubit Fluorometers

1. Allow all components of the Accuris ssDNA Quantification Kit to reach room temperature.

2. Inspect the Accuris ssDNA reagent for any precipitation. If small precipitates or particles are observed, warm the tube in a water bath until fully dissolved.

3. Prepare the required number of assay tubes, accounting for the two standards. (Use ONLY 0.5 mL thin-wall PCR tubes.)

4. Label the sample and standard tubes on the lids.

5. Prepare the working solution by diluting the Accuris ssDNA reagent 1:200 in 1X Accuris ssDNA Buffer immediately before use. For example, for eight samples plus two standards, dilute 10 μL of Accuris ssDNA Reagent in 1,990 μL of Accuris ssDNA Buffer.

6. Add 10 μL of Standard #1 and Standard #2 to the appropriately labeled tubes (see image below). 7. Thoroughly mix the samples and standards, then incubate the tubes at room temperature in the dark for 2-3 minutes.

Standards and Sample Measurements

Standard Curve:

1. On the Home screen, select Oligo.

2. Insert standard 1 into a reading chamber and select start symbol followed by pressing the read symbol.

3. After selecting concentration units, choose calibrate followed by Read Standards.

4. Replace standard tube 1 with standard 2 and select Read standards to obtain reading for the second standard.

5. Select Save to save the standard curve. Note: this curve may be re-used for subsequent sample readings. We recommend to prepare new standard curve each time assay is performed.

Sample Quantification:

1. On the fluorometer screen, enter the volume of the unknown sample, then select the Start icon.

2. Once the fluorometer completes the quantification, the sample concentration and Relative Fluorescence Units (RFU) will be displayed on the screen.

3. Remove the sample, insert the next sample into the test chamber, and select the Start icon.

4. Repeat Step 3 until all samples have been quantified. Note: At any point during quantification, the fluorometer screen can be swiped left to display all recorded concentrations from the current experiment, with options to print or export the results. Selecting the Calculator function allows users to calculate the molarity of the sample (note: the ssDNA length [length(bp) = lenght of ssDNA (bases)] must be entered for accurate quantification).

5. After completing the quantification process, turn off the fluorometer.

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Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples

Sensitive quantitation down to 0.1pg/ul ds DNA
Large color touch screen for programming and operation
Two models for single or 8-sample processing
Built-in reagent calculators for sample preparation
Compact and portable (11 x 6 inch footprint)

Smart-Q Specifications

Model	Smart-Q™ 100 Fluorometer	Smart-Q™ 800 Fluorometer	Smart-Q™ Mini 115V	Smart-Q™ Mini 230V
Display	7.0-inch, color touch screen	7.0-inch, color touch screen	No external display (instrument operation via iOS/Android phone or tablet)	No external display (instrument operation via iOS/Android phone or tablet)
Dynamic Range	5 orders of magnitude	4 orders of magnitude	5 orders of magnitude	5 orders of magnitude
Light Source	Blue and Red LED	Blue and Red LED	Blue and Red LED	Blue and Red LED
Excitation Wavelengths	Blue: 460 ± 20nm and Red: 625 ± 20nm	Blue: 460 ± 20nm and Red: 625 ± 20nm	Blue: 470 ± 15 nm Red: 620 ± 20 nm	Blue: 470 ± 15 nm Red: 620 ± 20 nm
Emission Wavelengths	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm	Green: 525 – 570nm and Red: 670 – 725nm
Stability	≤ 1.5%	≤ 1.5%	≤1.5%	≤ 1.5%
Detector	Photodiodes (300-1000nm)	Photodiodes (300-1000nm)	Photodiodes (320-1100nm)	Photodiodes (320-1100nm)
Measurement Speed	≤ 5 seconds	≤ 6 seconds	≤ 5 seconds	≤ 5 seconds
Consumable type	0.5mL assay tubes	0.2mL assay tubes (single and 8-tube strip)	0.5 mL optically-clear quantification tubes	0.5 mL optically-clear quantification tubes
Input Voltage	24 VDC 2A	24 VDC 2A	115V	230V
Dimensions (WxDxH)	16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in.	16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in.	3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm	3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm
Weight	1.6kg / 3.5lbs	2.0kg / 4.4lbs	0.5kg / 1.1lbs	0.5kg / 1.1lbs
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Description

	The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving: Next Generation Sequencing PCR Transfection Western blotting Immunoassays The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers. Intuitive Usability Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can be also be exported easily to a PC using a USB flash drive. Accurate Quantification The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements. The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations. The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits. Options for Throughput The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements: The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T). This renders the Smart-Q 100 ideal for laboratories processing fewer samples. The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ). The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples. Accuris DNA Quantification Kits Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, the sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument. High Sensitivity dsDNA Assay Kit The Accuris High Sensitivity kit provides an accurate method for the quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng Broad Range dsDNA Assay Kit The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng. ssDNA Quantification Kit The Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents RNA High Sensitivity Quantification Kit The Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration. This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL RNA Broad Range Quantification Kit The Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification. This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents


Smart-Q 100 for single sample processing

Smart-Q 800 for up to 8-sample processing

Accuris DNA Quantification Kits

Ordering Information

Smart-Q™	Cat.No.	Products	Qty ( Pack )	Order
	BM-F1001	Smart-Q 100 Fluorometer, single sample	1
	BM-F1008	Smart-Q 800 Fluorometer, 8-channel1	1
	BM-F0100	Smart-Q Mini Fluorometer, 115V	1
	BM-F0100-E	Smart-Q Mini Fluorometer, 230V	1
	BM-F0100-TAB	Android tablet preloaded with Smart-Q Mini app	1
	BM-F1000-HS-100	High Sensitivity dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-HS-500	High Sensitivity dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-HS1-100	1X High Sensitivity dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-HS1-500	1X High Sensitivity dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-BR-100	Broad Range dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-BR-500	Broad Range dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-BR1-100	1X Broad Range dsDNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-BR1-500	1X Broad Range dsDNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-SD-100	ssDNA Quantification Kit, 100 Assays	1 Kit
	BM-F1000-SD-500	ssDNA Quantification Kit, 500 Assays	1 Kit
	BM-F1000-RHS-100	High Sensitivity RNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-RHS-500	High Sensitivity RNA Quantification Kit, 500 assays	1 Kit
	BM-F1000-RBR-100	Broad Range RNA Quantification Kit, 100 assays	1 Kit
	BM-F1000-RBR-500	Broad Range RNA Quantification Kit, 500 assays	1 Kit	Current page
	BM-F1001-T	0.5mL Fluorescence Detection Tubes, pack of 250	250
	BM-F1008-T	0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000	1000
	BM-F1008-T-8	0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120	120