Bipo Resin Protein A (Alkaline Resistance)

Cat. No.:  FNK-AAR-025

Size: 25ml

Storage: 4℃

 

Specifications

Product Information:

Protein A (Staphylococcus aureus) is known as an affinity molecule for antibodies.

Use for purification of antibodies, small molecule antibodies (Fab, scFv), and immunoprecipitation.

25mL resin in 20% EtOH (50% slurry) (average particle size 60µm)

Alkaline Resistance: 28 (human IgG)


Antibody Purification Procedure

<Additional materials required>

• Empty spin column
• Binding buffer (PBS)
• Elution buffer (0.1M Glycine, pH2.5)*
• Neutralization buffer (1.0M Tris, pH9)

1) Equilibrate the resin

• Transfer resin (100µL) to empty column (column volume; 500µL).
• Centrifuge (3,000x 1min.) and discard the eluate.
• Add deionized water (400µL) to the column and centrifuge twice.
• Add PBS (400µL) to the column and centrifuge twice. 

2) Purification

• Transfer sample to column and incubate for 5min at room temperature and centrifuge.
• Add PBS (400µL) to the column and centrifuge three times.
• Add elution buffer (100µL) to the column and for 1-2min at room temperature and centrifuge twice.
• Add 5µL of neutralization buffer to each eluate.

Cleanup

100mM NaOH, 10min.

After clean up, NaOH should be replaced with deionized water and 20% EtOH immediately. Store at 4℃.