Benzonase Dilution Buffer [10X]
Catalog No.: DCP-BDB10X
Size: 500 mL
Sterile: Filtered 0.1-micron membrane TWICE, 0.04-micron membrane TWICE
pH: 7.5
Descriptions:
Benzonase Dilution Buffer is a specially formulated solution designed to dilute Benzonase nuclease enzyme to convenient working concentrations while maintaining its enzymatic activity and stability. The buffer provides the essential ionic environment and optimal pH necessary to preserve enzyme conformation during dilution. The buffer’s salt concentration balances enzyme stability without significantly inhibiting activity. Due to its composition, it supports enzyme function within a broad temperature range and is compatible with common laboratory conditions. Preparing the buffer with sterile water and handling under aseptic conditions makes it suitable for sensitive molecular biology applications such as nucleic acid contamination removal, sample preparation, and viscosity reduction in protein extracts.
Features:
- Compatible with common reagents and does not inhibit Benzonase at working concentrations.
- Facilitates easy dilution of concentrated Benzonase stocks for precision handling.
- Suitable for aseptic preparation, allowing use in sterile or contamination-sensitive workflows.
Composition:
| Ingredients |
Concentration |
| Tris-HCl (pH 7.5) |
200 mM |
| Sodium chloride |
100 mM |
| Magnesium chloride hexahydrate |
20 mM |
| Spermidine |
1 mM |
Quality Control
- Apperance: Clear Solution
- Sterility: Filtered in a sterile environment.
Storage and Shelf Life
- Storage: 4°C
- Shelf Life: 3 years, when properly sealed
