Anti-Sup35/PSI+ (S.cerevisiae) antibody, rabbit polyclonal IgG
Product#: FNK-62-300
$421.11
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Anti-Sup35/PSI+ (S.cerevisiae) antibody, rabbit polyclonal IgG
General information
| Cat. No. | ：FNK-63-300 |
| Size | ：100 ul |
| Antigen Species | ：Yeast |
| Host Species | ：Rabbit |
| Purification | ：Affinity Purified |
| Label | ：Unlabeled |
| Reactivity | ：S. cerevisiae Sup35, not tested with other species |
| Immunogen | ：Synthetic peptide corresponding to a.a. 494-507 of Sup35 |
| Product | ：Rabbit polyclonal antibody, purified IgG fraction |
| Application | ：Western blotting (1,000~2,000 fold dilution). Not tested for other applications. |
| Storage | ：-20℃ (for longer period, -70℃) |
| Form | ：Purified IgG 4 mg/ml in PBS, 50% glycerol, 0.09% sodium azide, |
| Data Link | ：SGD SUP35/YDR172W, UniProtKB P05453 (ERF3_YEAST) |
Description
Sup35 protein of S. cerevisiae is the translation termination factor eRF3. The altered conformation of this protein generates the [PSI+] prion phenotype . In this state, a dominant cytoplasmically inherited protein aggregates are formed which sequester the normal function of Sup35 thereby nonsense suppressor phenotype is created . The molecular chaperon Hsp104 is necessary for the formation and maintenance of the aggregates.
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Figure. Identification of Sup35 protein in crude extracts of S. cerevisiae by western blotting with anti-Sup35 antibody.
Anti-Sup35 antibody was used at 1/2,000 dilution. Sup35 protein was identified at 77 kDa position (Expected molecular mass of Sup35 is 76.6 kDa) . |
Reference
This antibody was used in the following ref.erence.
- Kimura Y et al “The role of pre-existing aggregates in Hsp104-dependent polyglutamine aggregate formation and epigenetic change of yeast prions” Genes to Cells 9: 685-696 (2004)
