Anti-Human Apolipoprotein E Antibody, (E-3D2) Biotinylated
Anti-Human Apolipoprotein E Antibody, (E-3D2) Biotinylated
DiagnoCine offers excellent APOLIPOPROTEIN antibodies to researchers studying human induced pluripotent stem cell, mitochondrial dysfunction, pathophysiology, genetic polymorphisms, metabolism, phospholipase A2 activity, cellular surface factor, angiotensin-converting enzyme, nitric oxide synthase activity, amyloidosis, epigenome/metabolome research, sialylation proteoforms, pathogenesis, and progression.
Human diseases include type 1 diabetes, Memory Deficit, hepatitis C virus, Alzheimer's Disease, Osteoporotic Fractures. endoplasmic reticulum stress, atherosclerotic lesions, cancer, multiple sclerosis, Toxic Hetero-oligomers, brain amyloid load, hypertriglyceridemic pancreatitis, Atherosclerosis, Schnauzers, coronary artery disease, psoriasis, cardiac amyloidosis, cardiovascular disease and hypertension, dementia, type 2 diabetes mellitus, toxicity in human neurons, cognitive impairment, atherothrombosis, familial hypobetalipoproteinemia, hyperlipidemia, Parkinson's disease, metabolic syndrome, coronary heart disease, and depression.
APOLIPOPROTEIN antibodies have excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization.
General information
| Cat. No. | ：FNK-BML012 |
| Size | ：100 µg |
| Formulation | ：0.2 µm filtered PBSsolution |
| Immunogen | ：human plasma VLDL |
| Specificity | ：This antibody has been selected for its ability to bind for human and rabbit apolipoprotein E by western blotting. |
| Ig Type | ：IgG2a |
| Clone | ：E-3D2 |
| Application | ：Western blotting, Sandwich ELISA |
| Cross Reactivity | ：Human/ Rabbit |
| Antigen | ：Human |
| Host Animal | ：Mouse |
| Purification | ：PAPu |
| Storage | ：IgG in PBS solution are stable for twelve months from the date of receipt when stored at-80˚C. Avoid repeated freeze-thaw cycles. |
Preparation
Produced in mice immunized with very low-density lipoprotein (VLDL) purified from human plasma. Apo E specific IgG was purified from mouse ascites fluid with a proteinA-Sepharose.
Additional Applications
Western Blot – Thai antibody can be used at 0.5 – 1.0 µg/mL with the appropriate secondary reagent to detect human and rabbit plasma apolipoprotein E (apoE). The detection limit for plasma apoE is approximately 0.05 µL,underreducing conditions.
Sandwich ELISA – This biotinylated antibody (Catalog #BML012) can be used as a detection antibody in a human apo E ELISAin combination with the monoclonal capture antibody (Catalog #BML010). A general protocol is provided on the next page. Using plates coated with 100 µL/well of the capture antibody, in combination with 100 µL/well of the detection antibody at 500 ng/mL, an ELISAfor sample volumes of 100 µL can be obtained. Titrate each preparation of the serum sample for standard preparation to arrive at the most suitable dose range.
Optimal dilutions should be determined by each laboratory for each application
Antigen Characterization
| Type | ：Protein |
| Application | ：ELISA, Western Blot, Immunohistochemistry (IHC), Flow Cytometry |
| Species | ：Human |
| Recombinant | ：Yes |
Apolipoprotein E in the Cell
- Modulates cytoskeletal assembly and stability
- Influences mitochondrial integrity and function
- Affects dendritic morphology and function in neurons
- Participates in intracellular lipid transport and metabolism
- Facilitates movement and processing of lipids within cells
Apolipoprotein E in the Human Body
- Serves as a key component of lipoproteins
- Plays a central role in lipid metabolism and transport
- Functions as a ligand for cell-surface receptors
- Mediates uptake and clearance of lipoproteins from the bloodstream
- Crucial for maintaining normal cholesterol levels
- Acts as the primary cholesterol carrier in the central nervous system
- Facilitates lipid transport between astrocytes and neurons
- Involved in immunoregulation
- Influences cognitive function
- Implicated in the pathogenesis of Alzheimer's disease
- Different ApoE isoforms (particularly ApoE4) associated with varying risk levels for Alzheimer's disease
Aliases for APOE Gene
- Apolipoprotein E 2 3 4 5
- Alzheimer Disease 2 (APOE*E4-Associated, Late Onset) 2
- Apolipoprotein E3 3
- LDLCQ5 3
- APO-E 3
- ApoE4 3
- Apo-E 4
- APOE 5
- AD2 3
- LPG 3
References
- Huang, Yadong, and Robert W Mahley. “Apolipoprotein E: structure and function in lipid metabolism, neurobiology, and Alzheimer's diseases.” Neurobiology of disease vol. 72 Pt A (2014): 3-12. doi:10.1016/j.nbd.2014.08.025
- Kim, Jungsu et al. “The role of apolipoprotein E in Alzheimer's disease.” Neuron vol. 63,3 (2009): 287-303. doi:10.1016/j.neuron.2009.06.026
- Alzheimer’s Disease Genetics Fact Sheet | National Institute on Aging, www.nia.nih.gov/health/alzheimers-causes-and-risk-factors/alzheimers-disease-genetics-fact-sheet.
- “APOE Gene: Medlineplus Genetics.” MedlinePlus, U.S. National Library of Medicine, medlineplus.gov/genetics/gene/apoe/.
- Yamazaki, Y., Zhao, N., Caulfield, T.R. et al. Apolipoprotein E and Alzheimer disease: pathobiology and targeting strategies. Nat Rev Neurol 15, 501–518 (2019). https://doi.org/10.1038/s41582-019-0228-7
- Raulin, AC., Doss, S.V., Trottier, Z.A. et al. ApoE in Alzheimer’s disease: pathophysiology and therapeutic strategies. Mol Neurodegeneration 17, 72 (2022). https://doi.org/10.1186/s13024-022-00574-4
- “APOE Apolipoprotein E [Homo Sapiens (Human)] - Gene - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/gene/348.
