Anti-HBs Pre-S2, Poly

Cat. No.: FNK-BCL-ABP2-01

Size: 100μg

Storage: 4℃ (for long term storage, -20℃. Avoid freeze and thaw cycles)


Introduction:

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection.




Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection and Pre-S2 is reportedly known to related HBV entry or carcinogenesis. Pre-S2 region is known to have variation of amino acid sequence depending on genotypes.

Specifications:

Source : white rabbit

Appearance : liquid form（PBS containing biochemically inactive preservative）

Concentration: 1 mg/mL

Antigen: Pre-S2 peptide with thioredoxin-tag（genotype C, FNK-BCL-AGS2C-02）

Purification: affinity by Protein A

Usage : ELISA (0.5 to 10μg/mL), Western blot (0.5 to 10μg/mL)


Antigen specificity: These antibodies bind only to Pre-S2 domain and not to S or Pre-S1 domains


Genotype specificity: 

The antibody binds to L-antigen of genotype A, B, C and D, but the relative affinity is different depending on genotype as shown in the figure.

Examples:

1. Western Blot

M；Marker 1. Antibody: anti-Pre-S1（Code＃ FNK-BCL-ABP1-01） Antigen: BNC-L 2. Antibody: anti-Pre-S2（Code＃ FNK-BCL-ABP2-01） Antigen: BNC-L 3. Antibody: anti-S antigen（Code＃ FNK-BCL-ABPS-01） Antigen: BNC-XT Monomer and dimer bands can be seen by the antibodies.

2. ELISA
 

ELISA condition Immobilized IgG: anti-Pre-S2 antibody (polyclonal, FNK-BCL-ABP2-01) at 10 μg/mL Blocking: k-Block-e (Code#: FNK-BCL-BKHE-01) Detection IgG: anti-Pre-S2 antibody (monoclonal, HRP-labeled) at 25 ng/mL Antigen: HBsAg L-protein (FNK-BCL-AGC-01)