Anti-HBs Pre-S2, Mono-2

Cat. No.: FNK-BCL-ABM2-02

Size: 100μg

Storage: 4℃ (for long term storage, -20℃. Avoid freeze and thaw cycles)


Introduction:

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection.




Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection and Pre-S2 is reportedly known to related HBV entry or carcinogenesis. Pre-S2 region is known to have variation of amino acid sequence depending on genotypes.

Specifications:

Source : mouse ascitic fluid, monoclonal
Appearance : liquid form（PBS containing biochemically inactive preservative）

Concentration: 1 mg/mL

Antigen: HBsAg L-protein

Purification: affinity by Protein G

Usage : ELISA (0.1 to 10μg/mL), Western blot (0.1 to 10μg/mL)


Antigen specificity: These antibodies bind only to Pre-S2 domain and not to S or Pre-S1 domains


Genotype specificity: 


FNK-BCL-ABM2-01 mono-1 binds to L antigen of all genotypes examined.

FNK-BCL-ABM2-02 mono-2 binds to L antigen of genotype A,B,C but not that of genotype B.



Examples:

1. Western Blot

Figure shows results of western blot using these antibodies using HBsAg-L protein as a sample. Antigen: HBsAg-Lprotein, 10ng Primary antibody: -Lane1: Anti-Pre-S2 antibody mono-1 (FNK-BCL-ABM2-01) at 0.1μg/mL -Lane2: Anti-Pre-S2 antibody mono-2 (FNK-BCL-ABM2-02) at 0.1μg/mL Secondary antibody: Anti-mouse IgG Rabbit (HRP -labeled) Detection: 1 min exposure

2. ELISA

Figure shows results of ELISA using these antibodies using HBsAg-L protein ST type as a sample. Solid Phase: Anti-Pre-S2 polyclonal antibody (FNK-BCL-ABP2-01) 10 μg/mL Blocking: h-Block-e (FNK-BCL-BKHE-01) Antigen: HBsAg-Lprotein ST type Detection: Anti-Pre-S2 antibody mono-1 (FNK-BCL-ABM2-01), HRP-labeled at 1 μg/mL