Anti-FcεR1α（human IgE receptor）monoclonal antibody (CRA1), FITC-labeled

Anti-FcεR1α（human IgE receptor）monoclonal antibody (CRA1), FITC-labeled 
 

DiagnoCine offers excellent Anti-FcεR1α | Human IgE (Immunoglobin E) receptor antibodies  to researchers studying Allergic rhinitis treatment, Intranasal antihistamines, Intranasal corticosteroids, Leukotrienes, Subcutaneous immunotherapy, and Mechanisms & Treatment of Allergy.

Human diseases include Seasonal rhinitis, Atopic dermatitis, Anaphylaxis, Asthma, Dermatographia, and Physical Urticaria.

Anti-FcεR1α | Human IgE (Immunoglobin E) receptor antibody has excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization.  

General Information

Description 

FcεR1α is a subunit of the high-affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α, one β, and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses.

The CRA1 (AER37) monoclonal antibody reacts with the FcεR1α subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1α, it competes with IgE for receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1α.

The IgG fraction was purified from a serum-free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. This product is a FITC-labeled IgG ([FITC]/[IgG] = 5.0) produced from the IgG fraction.
 

Figure: FACS analysis of CHO/αβγ cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining using FITC-labeled secondary antibody.

References 

1. Ra C et al “A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit”Nature 341:752-754 (1989) PMID: 2529442
2. Hakimi J et al “The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding” J Biol Chem 265:22079-22089 (1990) PMID: 2148316
3. Yamaguchi M et al ”IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release”J Immuno1 162:5455-5465 (1999) PMID: 10228025
4. Suzukawa M et al “IgE- and FcepsilonRI-mediated migration of human basophils” Int Immunol 17 : 1249-1255 (2005) PMID: 16103029
5. Takai T et al “Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions” Biosci Biotechnol Biochem64:1856-1867(2000) PMID: 11055388.
6. Takai T et al “Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli ”Biosci Biotechnol Biochem 65:79-85 (2001) PMID: 11272849
7. Untersmayr E et al “The High Affinity IgE Receptor FceRI Is Expressed by Human Intestinal Epithelial Cells” PLoS ONE 5 (2):1-11 (2010) PLoS ONE: 0009023

Aliases for FCER1A Gene

• Fc Fragment Of IgE Receptor Ia ^2 3 5
• Fc Fragment Of IgE, High Affinity I, Receptor For; Alpha Polypeptide ^2 3
• High Affinity Immunoglobulin Epsilon Receptor Subunit Alpha ^3 4
• IgE Fc Receptor Subunit Alpha ^3 4
• Fc Epsilon Receptor Ia ^2 3
• Fc-Epsilon RI-Alpha ^3 4
• FCE1A ^3 4
• FcERI ^3 4
• Immunoglobulin E Receptor, High-Affinity, Of Mast Cells, Alpha Polypeptide ³
• High Affinity Immunoglobulin Epsilon Receptor Alpha-Subunit ³
• Fc IgE Receptor, Alpha Polypeptide ³
• Fc Epsilon RI Alpha-Chain ³
• FCER1A ⁵