Agarose Pre-Made Gel in 0.5X TAE, with SSG

Product#: GA6030
$154.51
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Agarose Pre-Made Gel in 0.5X TAE, with SSG

Cat.No.   GA6030
Storage: 4~8℃ / Protect from light.
Shelf Life: 8 months

*We recommend you wear personal protective equipment (such as protective clothing and gloves) and you clean your hands after use.
*Keep the product protected from shock.
*Keep the product from being frozen.

Product Description
Agarose gel refers to a gel substance created by melting polysaccharide in hot water, which is the main ingredient of agar that does not include  sulfate, and then letting it cool. As it is used for the electrophoresis for high-molecularmass nucleotides, it serves as a medium through which DNA or RNA moves. As it has the excellent ability to fix DNA or RNA, it is effective for separation by molecular mass.

It is a pre-made agarose gel that includes Safe Shine Green. EtBr, which is widely used, is a reagent that registers a strong toxicity known as mutagen and carcinogen. Safe Shine Green, which is a replacement for EtBr, is a non-toxic, non-mutagenic and non-carcinogenic stable fluorescence dye. By comparing EtBr with Safe Shine Green by DNA concentration, one can see that Safe Shine Green is similar to EtBr in sensitivity.

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Comparison of EtBr and Safe Shine Green (SSG) in precasting gel staining using 2% agarose gel in TAE buffer. Serial dilution of 100 bp DNA Ladder were loaded in the amount of 650 ng. 390 ng. 130 ng, 65 ng, 32.5 ng and 13 ng from left to right. Gels were imaged using UV transilluminator and photographed with 590 nm and 520 nm. 

Note
Safe Shine Green registers a maximum fluorescent wavelength of 535㎚ (below), and you can obtain optimum images if you use a fluorescent filter at the same wavelength. However, as the work can be done with a UV filter alone, it can be used without any extra equipment.
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Pre-made Agarose Gel for Multi-Pipette and Screening

12 well gel.png      For Multi-Pipette (12 well)
Gel Size W: 110 mm, H: 63 mm / Max. Volume: 22 uL

96 well gel.png      For Screening (96 well)
Gel Size W: 115 mm, H: 120 mm / Max. Volume: 20 uL / 25 mm

Caution: EtBr, which is toxic not only as a potent carcinogen, but also as a mutagen. So, when handling this product, you must wear personal protective equipment including gloves.

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