ALPQuench-PBS

Cat No.: DCP-HRPTBS1X

Size: 100 mL

Non-Sterile; Ultrapure, Filtered 0.1-micron membrane ONCE and 0.04-micron membrane ONCE.

ALPQuench-PBS represents a significant advancement in alkaline phosphatase (ALP) signal management for immunoassays, delivering precise control in sequential and multiplex immunostaining protocols. This innovative phosphate-buffered saline-based solution, formulated with a proprietary blend of enzyme inhibitors and stabilizers, effectively suppresses ALP activity between multiple labeling steps without compromising sample integrity or subsequent antibody binding. By eliminating cross-reactivity and non-specific signals, ALPQuench-PBS significantly enhances the specificity and reliability of complex immunohistochemistry and ELISA procedures. Especially valuable for protocols involving multiple ALP-conjugated antibodies, it enables clear, distinct labeling of different antigens within the same specimen. Its optimized formulation not only improves staining quality but also streamlines workflows, reducing overall experiment time and reagent consumption, making it an essential tool for advanced immunological research.

Applications:

• Multiplex immunohistochemistry (mIHC) using sequential ALP-based staining cycles

• Multi-round immunofluorescence protocols requiring ALP inactivation between labeling steps

• ELISA procedures involving multiple ALP-conjugated antibodies or detection reagents

• Endogenous alkaline phosphatase suppression in tissues with high background activity (e.g., intestine, liver, kidney)

• Multi-round IHC protocols using same-species primary antibodies with ALP-conjugated secondaries

• Automated IHC/IF staining platforms requiring standardized ALP quenching steps

Advantages:

• Complete ALP Inactivation — effectively suppresses residual alkaline phosphatase activity between staining rounds, preventing signal carryover and cross-reactivity

• Sample Integrity Preserved — does not compromise tissue morphology or disrupt subsequent antibody-antigen interactions

• Cleaner Results — eliminates non-specific ALP-driven substrate reactions, reducing background in both chromogenic and fluorescent readouts

• PBS-Optimized — formulated in phosphate-buffered saline for broad compatibility with standard immunoreagents and downstream assay components

• Easy to Use — straightforward integration into existing protocols with no specialized equipment required

• Versatile — suitable for use on FFPE and frozen tissue sections as well as ELISA-based platforms

• Workflow Efficiency — reduces total assay time and the number of additional blocking steps needed across multi-round labeling protocols

Composition:
 

Quality Control:

• Appearance: Clear, Colorless Liquid.

• pH: 7.4

• Sterility: Non-Sterile

Storage and Shelf Life:

• Storage: 4℃ 

• Shelf life: 1 year

CUSTOMIZATION:  Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.

* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.