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6-Phosphogluconate Dehydrogenase, Thermostable

Product#: FNK-PGD-75-01

$241.17

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6-Phosphogluconate Dehydrogenase, Thermostable

Cat. No. FNK-PGD-75-01
Stroage -20°C
Size 1ml
Lot No. 1-H003A
Protein conc. 0.32 mg/ml
Form 20 mM Tris-HCl (pH 7.5)
Activity 10.3 U/ml
Notes For research use only

EC No.1.1.1.44
Substrate 6-phosphogluconate
Reaction product ribulose 5-phosphate + CO2
Reaction temperature : ~70℃
Reaction pH 6.0~9.0

Description

Enzymes work at high temperature with high reactivity The "Thermostable Enzyme".

Generally, enzymes in the living body are denatured and lose activities at the temperature over 50 - 60°C.
However, the thermostable enzymes obtained from special microorganisms (hyper-thermophilic microorganisms) alive at high temperature (over 70°C) have unique characteristics.
Using regular enzymes as a catalyst in chemical reactions are not cost effective due to its low stability and needs of additional enzyme.
By using these thermostable enzymes, we can expect not only cost saving but also other advantages - especially in the field of fine chemicals.

Features of Thermostable Enzyme

Highly stable and durable for long term use
High reactivity at high temperature (70 - 100°C)
Resistance to organic solvent is expected
Special instruments to inhibit bacterial growth are not required.
Reaction speficity is much higher compared with chemical reaction
Speed of reaction can be accelerated

Activity measurement

Reaction mix (50 mM PIPES-KOH (pH 6.5), 1 mM 6-Phosphogluconate, 10 mM MgCl2, 0.3 mM NADP+ and appropriate amount of the enzyme) was incubated at 65 oC and A340 was monitored. One unit is defined as the amount of enzyme producing 1 µmol of NADPH (e340=6.22 mM^-1cm^-1 ) per 1 minute using 6-Phosphogluconate as a substrate.